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Literature summary extracted from
- D-Altronate: NAD-oxidoreductase in Escherichia coli K12. Purification, properties, and specificity (1972), Eur. J. Biochem., 26, 50-61.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 1.1.1.58 | altronate protects against heat denaturation | Escherichia coli |
| 1.1.1.58 | fructuronate does not protect against heat denaturation | Escherichia coli |
| 1.1.1.58 | mannonate protects against heat denaturation | Escherichia coli |
| 1.1.1.58 | NAD+ does not protect against heat denaturation | Escherichia coli |
| 1.1.1.58 | NADH protects against heat denaturation | Escherichia coli |
| 1.1.1.58 | NADPH protects against heat denaturation | Escherichia coli |
| 1.1.1.58 | tagaturonate does not protect against heat denaturation | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.58 | ATP | - |
Escherichia coli |  |
| 1.1.1.58 | additional information | products inhibition with all products in forward and reverse reaction | Escherichia coli | |
| 1.1.1.58 | p-chloromercuribenzoate | non-competitive, Ki: 1 mM | Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.58 | 0.06 | - |
NADH | - |
Escherichia coli | |
| 1.1.1.58 | 0.11 | - |
NAD+ | - |
Escherichia coli | |
| 1.1.1.58 | 0.35 | - |
tagaturonate | - |
Escherichia coli | |
| 1.1.1.58 | 0.4 | - |
NADPH | - |
Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.58 | D-altronate + NAD+ | Escherichia coli | - |
D-tagaturonate + NADH | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.58 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.58 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.58 | 6.2 | - |
- |
Escherichia coli |
| 1.1.1.58 | 328 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.58 | 4°C or -20°C, several months | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.58 | D-altronate + NAD+ | - |
Escherichia coli | D-tagaturonate + NADH | - |
r | |
| 1.1.1.58 | D-tagaturonate + NADPH + H+ | - |
Escherichia coli | D-altronate + NADP+ | - |
r | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.58 | 6.3 | - |
NADH oxidation | Escherichia coli |
| 1.1.1.58 | 8.9 | - |
NAD+ reduction | Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.58 | 6 | 8 | at pH 6 and 8: about 50% of activity maximum | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.58 | additional information | no activity with NADP+ | Escherichia coli | |
| 1.1.1.58 | NAD+ | - |
Escherichia coli | |
| 1.1.1.58 | NADH | - |
Escherichia coli | |
| 1.1.1.58 | NADPH | - |
Escherichia coli | |
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Release 2023.1 (January 2023)
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