Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Portalier, R.C.; Stoeber, F.R.
    D-Mannonate: NAD oxidoreductase from Escherichia coli K12. Purification, properties and specificity (1972), Eur. J. Biochem., 26, 290-300.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.57 ATP non-competitive Escherichia coli
1.1.1.57 D-altronate competitive at low concentrations; non-competitive Escherichia coli
1.1.1.57 additional information product inhibition with all products in forward and reverse reaction Escherichia coli
1.1.1.57 p-chloromercuribenzoate complete inhibition at 0.005 mM, non-competive, Ki: 0.0001 mM Escherichia coli
1.1.1.57 tagaturonate competitive, inhibitory on forward reaction Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.57 0.5
-
 D-fructuronate
-
 Escherichia coli
1.1.1.57 0.9
-
 D-tagaturonate
-
 Escherichia coli
1.1.1.57 1.8
-
 D-mannonate
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.57 D-mannonate + NAD+ Escherichia coli
-
 D-fructuronate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.57 Escherichia coli
-
 K 12
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.57
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.57 6
-
 crude extract Escherichia coli
1.1.1.57 320
-
 purified enzyme Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.57 D-altronate + NAD+
-
 Escherichia coli D-tagaturonate + NADH
-
 ?
1.1.1.57 D-mannonate + NAD+
-
 Escherichia coli D-fructuronate + NADH
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.57 53
-
 loss of activity, NADH, NADPH, fructuronate, tagaturonate, NAD+, mannonate and altronate protect against inactivation Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.57 6.3
-
 for NADH oxidation Escherichia coli
1.1.1.57 8.4
-
 for NAD reduction Escherichia coli