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Literature summary extracted from

  • Krook, M.; Prozorovski, V.; Atrian, S.; Gonzalez-Duarte, R.; Jörnvall, H.
    Short-chain dehydrogenases. Proteolysis and chemical modification of prokaryotic 3alpha/20beta-hydroxysteroid, insect alcohol and human 15-hydroxyprostaglandin dehydrogenase (1992), Eur. J. Biochem., 209, 233-239.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.53 sensitive to proteolysis with Glu-C protease and trypsin, which can be nearly completly hindered by presence of NADH or cortisone Streptomyces exfoliatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.53 additional information no inhibition with iodoacetate, acetylsialic acid, phenylmethylsulfonyl fluoride, glyoxal, iodoacetamide, dimethylaminoisopropyl ethylene dicarbodiimide, acetylimidazol, diethyl pyrocarbonate Streptomyces exfoliatus
1.1.1.53 pyridoxal 5'-phosphate slightly Streptomyces exfoliatus
1.1.1.53 Tetranitromethane other Tyr-selective reagents, Tyr residues are labeled with nitro-group by inhibitor Streptomyces exfoliatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.53 3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H Streptomyces exfoliatus enzyme belongs to the group of short-chain dehydrogenases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.53 Streptomyces exfoliatus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.53 3alpha,20beta-hydroxysteroid androstanes or pregnane derivatives + NAD(P)H enzyme belongs to the group of short-chain dehydrogenases Streptomyces exfoliatus ?
-
 ?
1.1.1.53 cortisone + NAD(P)H
-
 Streptomyces exfoliatus 17alpha,20beta,21-trihydroxy-pregn-4-ene-3,11-dione + NAD(P)+
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.53 additional information
-
 low stability Streptomyces exfoliatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.53 NADH protects enzyme almost completely against proteolysis with Glu-C protease and trypsin Streptomyces exfoliatus