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Literature summary extracted from

  • Schultz, R.M.; Groman, E.V.; Engel, L.L.
    3(17)beta-Hydroxysteroid dehydrogenase of Pseudomonas testosteroni. A convenient purification and demonstration of multiple molecular forms (1977), J. Biol. Chem., 252, 3775-3783.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.51 cytosol
-
 Comamonas testosteroni 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.51 98500
-
 native PAGE Comamonas testosteroni

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.51 Comamonas testosteroni
-
 ATCC 11996
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.51
-
 Comamonas testosteroni

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.51
-
 Comamonas testosteroni

Storage Stability

EC Number Storage Stability Organism
1.1.1.51 -20°C, 5 mM potassium phosphate buffer, pH 7.5, 1 mM dithiothreitol, 50% glycerol, at least 7 months Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.51 testosterone + NAD(P)+
-
 Comamonas testosteroni androst-4-en-3,17-dione + NAD(P)H + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.51 tetramer A3B, 4 * 23500-26700, SDS-PAGE, nonidentical subunits, similar in size, different in charge Comamonas testosteroni