BRENDA - Enzyme Database show

Isolation and characterization of 17beta-hydroxy steroid dehydrogenase from human erythrocytes

Mulder, E.; Lamers-Stahlhofen, G.J.M.; van der Molen, H.J.; Biochem. J. 127, 649-659 (1972)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.51
albumin
-
Homo sapiens
1.1.1.51
chloromercuribenzene-p-sulfonic acid
-
Homo sapiens
1.1.1.51
p-chloromercuribenzoate
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.51
0.17
-
testosterone
-
Homo sapiens
1.1.1.51
0.24
-
testosterone
NADP+ as variable substrate
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.51
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.51
64000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.51
17-oxo steroids + NADPH
Homo sapiens
maintenance of balance between oxidized and reduced steroids in blood
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.51
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.51
partial
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.51
erythrocyte
-
Homo sapiens
-
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.51
-15°C, a few percent loss of activity in several months
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.51
17-oxo steroids + NADPH
maintenance of balance between oxidized and reduced steroids in blood
287072
Homo sapiens
?
-
-
-
-
1.1.1.51
20alpha-hydroxypregn-4-en-3-one + NADP+
-
287072
Homo sapiens
progesterone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
androst-5-ene-3beta,17beta-diol + NADP+
-
287072
Homo sapiens
dehydroepiandrosterone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
estradiol + NADP+
-
287072
Homo sapiens
estrone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
estradiol sulfate + NADP+
-
287072
Homo sapiens
estrone sulfate + NADPH
-
287072
Homo sapiens
?
1.1.1.51
testosterone + NAD(P)+
-
287072
Homo sapiens
androst-4-en-3,17-dione + NAD(P)H + H+
-
287072
Homo sapiens
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.51
NADP+
specific for
Homo sapiens
1.1.1.51
NADPH
specific for
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.51
NADP+
specific for
Homo sapiens
1.1.1.51
NADPH
specific for
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.51
albumin
-
Homo sapiens
1.1.1.51
chloromercuribenzene-p-sulfonic acid
-
Homo sapiens
1.1.1.51
p-chloromercuribenzoate
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.51
0.17
-
testosterone
-
Homo sapiens
1.1.1.51
0.24
-
testosterone
NADP+ as variable substrate
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.51
cytosol
-
Homo sapiens
5829
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.51
64000
-
gel filtration
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.51
17-oxo steroids + NADPH
Homo sapiens
maintenance of balance between oxidized and reduced steroids in blood
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.51
partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.51
erythrocyte
-
Homo sapiens
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.51
-15°C, a few percent loss of activity in several months
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.51
17-oxo steroids + NADPH
maintenance of balance between oxidized and reduced steroids in blood
287072
Homo sapiens
?
-
-
-
-
1.1.1.51
20alpha-hydroxypregn-4-en-3-one + NADP+
-
287072
Homo sapiens
progesterone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
androst-5-ene-3beta,17beta-diol + NADP+
-
287072
Homo sapiens
dehydroepiandrosterone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
estradiol + NADP+
-
287072
Homo sapiens
estrone + NADPH
-
287072
Homo sapiens
r
1.1.1.51
estradiol sulfate + NADP+
-
287072
Homo sapiens
estrone sulfate + NADPH
-
287072
Homo sapiens
?
1.1.1.51
testosterone + NAD(P)+
-
287072
Homo sapiens
androst-4-en-3,17-dione + NAD(P)H + H+
-
287072
Homo sapiens
r