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Literature summary extracted from

  • Wennekes, L.M.J.; Goosen, T.; Van den Broek, P.J.M.; Van den Broek, H.W.J.
    Purification and characterization of glucose-6-phosphate dehydrogenase from Aspergillus niger and Aspergillus nidulans (1993), J. Gen. Microbiol., 139, 2793-2800.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.02
-
 NADPH
-
 Aspergillus nidulans
1.1.1.49 0.026
-
 NADP+
-
 Aspergillus niger
1.1.1.49 0.03
-
 NADP+
-
 Aspergillus nidulans
1.1.1.49 0.092
-
 D-glucose 6-phosphate
-
 Aspergillus nidulans
1.1.1.49 0.153
-
 D-glucose 6-phosphate
-
 Aspergillus niger

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 53000
-
 ? * 55000 + ? * 53000, SDS-PAGE Aspergillus niger
1.1.1.49 55000
-
 ? * 55000 + ? * 53000, SDS-PAGE Aspergillus niger
1.1.1.49 57000
-
 ? * 60000 + ? * 57000, SDS-PAGE Aspergillus nidulans
1.1.1.49 60000
-
 ? * 60000 + ? * 57000, SDS-PAGE Aspergillus nidulans
1.1.1.49 160000
-
 gel filtration Aspergillus niger
1.1.1.49 180000
-
 gel filtration Aspergillus nidulans

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Aspergillus nidulans
-
-
-
1.1.1.49 Aspergillus niger
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49
-
 Aspergillus nidulans
1.1.1.49
-
 Aspergillus niger

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 745
-
 purified enzyme Aspergillus nidulans
1.1.1.49 790
-
 purified enzyme Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + H2O + NADP+
-
 Aspergillus nidulans D-gluconate 6-phosphate + NADPH + H+
-
 r
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Aspergillus niger 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 r
1.1.1.49 additional information no activity with glucose, glucose 1-phosphate, 2-deoxyglucose 6-phosphate, fructose, fructose 6-phosphate, fructose 1,6-diphosphate, mannose, galactose 6-phosphate, NAD+ Aspergillus nidulans ?
-
 ?
1.1.1.49 additional information no activity with glucose, glucose 1-phosphate, 2-deoxyglucose 6-phosphate, fructose, fructose 6-phosphate, fructose 1,6-diphosphate, mannose, galactose 6-phosphate, NAD+ Aspergillus niger ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.49 ? ? * 55000 + ? * 53000, SDS-PAGE Aspergillus niger
1.1.1.49 ? ? * 60000 + ? * 57000, SDS-PAGE Aspergillus nidulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 7 9
-
 Aspergillus nidulans
1.1.1.49 7 9
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+ absolute specificity Aspergillus nidulans
1.1.1.49 NADP+ absolute specificity Aspergillus niger