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Literature summary extracted from
- D-Galactose dehydrogenase from Pseudomonas saccharophila. Purification, properties and structure (1973), Eur. J. Biochem., 40, 49-61.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.48 | 0.14 | - |
NAD+ | - |
Pelomonas saccharophila |  |
| 1.1.1.48 | 1 | - |
D-galactose | - |
Pelomonas saccharophila | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.48 | 25000 | - |
alpha4, 4 * 25000, SDS-PAGE | Pelomonas saccharophila |
| 1.1.1.48 | 102000 | - |
- |
Pelomonas saccharophila |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.48 | D-galactose + NAD+ | Pelomonas saccharophila | - |
D-galactono-1,5-lactone + NADH | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.48 | Pelomonas saccharophila | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 1.1.1.48 | oxidation of sulhydryl residues inactivates enzyme, reactivation with 100 mM 2-mercaptoethanol or 10 mM dithiothreitol, pH 7.0, 30°C, enzymatically active oligomers of native enzyme are formed upon storage without reducing agents | Pelomonas saccharophila |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.48 | ammonium sulfate, heat, phosphate precipitation, DEAE-cellulose, hydroxyapatite | Pelomonas saccharophila |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.48 | 202 | - |
- |
Pelomonas saccharophila |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.1.1.48 | 4°C, 3.5 M (NH4)2SO4, 20 mM 2-mercaptoethanol, pH 7.0, several months, no loss of activity | Pelomonas saccharophila |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.48 | 2-deoxy-D-galactose + NAD+ | - |
Pelomonas saccharophila | 2-deoxy-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.48 | 3-deoxy-D-galactose + NAD+ | - |
Pelomonas saccharophila | 3-deoxy-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.48 | 4-deoxy-D-galactose + NAD+ | - |
Pelomonas saccharophila | 4-deoxy-galactono-1,5-lactone + NAD(P)H | - |
? | |
| 1.1.1.48 | 6-deoxy-alpha-D-galactose + NAD+ | - |
Pelomonas saccharophila | 6-deoxy-galactono-1,5-lactone + NADH | - |
? | |
| 1.1.1.48 | D-galactose + NAD+ | - |
Pelomonas saccharophila | D-galactono-1,5-lactone + NADH | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.48 | tetramer | alpha4, 4 * 25000, SDS-PAGE | Pelomonas saccharophila |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.48 | 20 | 65 | 5 min, 100% activity at 57°C, rapid drop above 60°C | Pelomonas saccharophila |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.48 | 358 | - |
D-galactose | - |
Pelomonas saccharophila | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.48 | 8 | 9 | - |
Pelomonas saccharophila |
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