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Literature summary extracted from

  • Carlsson, H.; Ljungcrantz, P.; Lindbladh, C.; Persson, M.; Bulow, L.
    Use of geneticallly prepared enzyme conjugates in lactose and galactose analyses (1994), Anal. Biochem., 218, 278-283.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.48 analysis determination of lactose using a biosensor made of immobilized beta-galctosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.48 0.185
-
 D-galactose pH 7.5 Pseudomonas fluorescens
1.1.1.48 0.205
-
 D-galactose pH 8.0 Pseudomonas fluorescens
1.1.1.48 0.215
-
 L-arabinose pH 7.5 Pseudomonas fluorescens
1.1.1.48 0.255
-
 D-galactose pH 8.5 Pseudomonas fluorescens
1.1.1.48 0.26
-
 D-galactose pH 7.5, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens
1.1.1.48 0.275
-
 L-arabinose pH 8.0 Pseudomonas fluorescens
1.1.1.48 0.32
-
 D-galactose pH 8.0, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens
1.1.1.48 0.37
-
 L-arabinose pH 8.5 Pseudomonas fluorescens
1.1.1.48 0.43
-
 L-arabinose pH 7.5, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens
1.1.1.48 0.43
-
 D-galactose pH 8.5, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens
1.1.1.48 0.5
-
 L-arabinose pH 8.0, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens
1.1.1.48 0.68
-
 L-arabinose pH 8.5, beta-galactosidase-galactose dehydrogenase fusion enzyme Pseudomonas fluorescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.48 D-galactose + NAD+ Pseudomonas fluorescens
-
 D-galactono-1,5-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.48 Pseudomonas fluorescens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.48 D-galactose + NAD+
-
 Pseudomonas fluorescens D-galactono-1,5-lactone + NADH
-
 ?
1.1.1.48 L-arabinose + NAD+
-
 Pseudomonas fluorescens L-arabinono-1,4-lactone + NADH
-
 ?