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Literature summary extracted from

  • Novick, N.J.; Tyler, M.E.
    Partial purification and properties of an L-arabinose dehydrogenase from Azospirillium brasilense (1983), Can. J. Microbiol., 29, 242-246.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.376 CoCl2 10 mM, 54% inhibition Azospirillum brasilense
1.1.1.376 dithiothreitol 1 mM, 10% inhibition Azospirillum brasilense
1.1.1.376 EDTA 2.5 mM, 8% inhibition Azospirillum brasilense
1.1.1.376 FeSO4 10 mM, 30% inhibition Azospirillum brasilense
1.1.1.376 MnCl2 10 mM, 45% inhibition Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.376 0.075
-
 L-arabinose pH 9,0, 25°C, cofactor: NADP+ Azospirillum brasilense
1.1.1.376 0.14
-
 L-arabinose pH 9.0, 25°C, cofactor: NAD+ Azospirillum brasilense

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.376 additional information no enhancement of activity in presence of divalent cations Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.376 175000
-
 gel filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.376 Azospirillum brasilense
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.376 partial Azospirillum brasilense

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.376 2.6
-
 pH 9.0, 25°C, L-arabinose + NADP+ Azospirillum brasilense
1.1.1.376 4.1
-
 pH 9.0, 25°C, L-arabinose + NAD+ Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.376 L-arabinose + NAD+
-
 Azospirillum brasilense L-arabinono-1,4-lactone + NADH + H+
-
 ?
1.1.1.376 L-arabinose + NADP+
-
 Azospirillum brasilense L-arabinono-1,4-lactone + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.376 L-arabino-aldose dehydrogenase
-
 Azospirillum brasilense

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.376 25
-
 assay at Azospirillum brasilense

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.376 55
-
 stable for 5 min Azospirillum brasilense

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.376 9.5
-
-
 Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.376 NAD+
-
 Azospirillum brasilense
1.1.1.376 NADP+
-
 Azospirillum brasilense

General Information

EC Number General Information Comment Organism
1.1.1.376 metabolism first enzyme in metabolism of L-arabinose Azospirillum brasilense