Literature summary extracted from

Veronese, F.M.; Boccu, E.; Schiavon, O.; Grandi, C.; Fontana, A.
General stability of thermophilic enzymes: Studies on 6-phosphogluconate dehydrogenase from Bacillus stearothermophilus and yeast (1984), J. Appl. Biochem., 6, 39-47.

General Stability

EC Number	General Stability	Organism
1.1.1.44	remarkably stable to the action of trypsin, chymotrypsin and elastase	Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
1.1.1.44	100000	-	-	Geobacillus stearothermophilus

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.1.1.44	6-phospho-D-gluconate + NADP+	Saccharomyces cerevisiae	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
1.1.1.44	6-phospho-D-gluconate + NADP+	Geobacillus stearothermophilus	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
1.1.1.44	6-phospho-D-gluconate + NADP+	Geobacillus stearothermophilus NCA 1503	-	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Organic Solvent Stability

EC Number	Organic Solvent	Comment	Organism
1.1.1.44	Acetone	enzyme shows 15-20% enhanced activity	Geobacillus stearothermophilus
1.1.1.44	dimethylformamide	enzyme shows 15-20% enhanced activity	Geobacillus stearothermophilus
1.1.1.44	dioxane	enzyme shows 15-20% enhanced activity	Geobacillus stearothermophilus

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.1.1.44	Geobacillus stearothermophilus	-	NCA 1503	-
1.1.1.44	Geobacillus stearothermophilus NCA 1503	-	NCA 1503	-
1.1.1.44	Saccharomyces cerevisiae	-	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.1.1.44	6-phospho-D-gluconate + NADP+	-	Saccharomyces cerevisiae	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
1.1.1.44	6-phospho-D-gluconate + NADP+	-	Geobacillus stearothermophilus	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r
1.1.1.44	6-phospho-D-gluconate + NADP+	-	Geobacillus stearothermophilus NCA 1503	D-ribulose 5-phosphate + CO2 + NADPH + H+	-	r

Subunits

EC Number	Subunits	Comment	Organism
1.1.1.44	dimer	-	Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
1.1.1.44	48	-	-	Saccharomyces cerevisiae
1.1.1.44	67	-	-	Geobacillus stearothermophilus

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Release 2023.1 (January 2023)