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Literature summary extracted from

  • Dyson, J.E.D.; D'Orazio, R.E.; Hanson, W.H.
    Sheep liver 6-phosphogluconate dehydrogenase: Isolation procedure and effect of pH, ionic strength, and metal ions on the kinetic parameters (1973), Arch. Biochem. Biophys., 154, 623-635.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.44 0.00571
-
 NADP+ 10 mM Tris-acetate, 10 mM MgCl2, pH 7.7 Ovis aries
1.1.1.44 0.00631
-
 NADP+ 10 mM Tris-acetate, 80 mM KCl, pH 7.7 Ovis aries
1.1.1.44 0.00676
-
 NADP+ 50 mM Tris-acetate, pH 7.7 Ovis aries
1.1.1.44 0.00861
-
 NADP+ 50 mM Tris-acetate, 20 mM MgCl2, pH 7.7 Ovis aries
1.1.1.44 0.00893
-
 NADP+ 10 mM Tris-acetate, pH 7.7 Ovis aries
1.1.1.44 0.0149
-
 6-phosphogluconate 50 mM Tris-acetate, 5-10 mM MgCl2, pH 7.7 Ovis aries
1.1.1.44 0.015
-
 6-phosphogluconate
-
 Ovis aries
1.1.1.44 0.016
-
 NADP+
-
 Ovis aries
1.1.1.44 0.0161
-
 6-phosphogluconate 50 mM Tris-acetate, pH 7.7 Ovis aries
1.1.1.44 0.0167
-
 6-phosphogluconate 10 mM Tris-acetate, 80 mM KCl, pH 7.7 Ovis aries
1.1.1.44 0.0413
-
 6-phosphogluconate 10 mM Tris-acetate, pH 7.7 Ovis aries
1.1.1.44 0.05
-
 6-phosphogluconate 50 mM Tris-acetate, 20 mM MgCl2, KCl, pH 7.7 Ovis aries

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+ Cyberlindnera jadinii
-
 D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Rattus norvegicus enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ Ovis aries enzyme specific for the oxidative pentose phosphate pathway D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.44 Cyberlindnera jadinii
-
-
-
1.1.1.44 Ovis aries
-
 sheep
-
1.1.1.44 Rattus norvegicus
-
 rat
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.44 liver
-
 Rattus norvegicus
-
1.1.1.44 liver
-
 Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.44 17.4
-
-
 Ovis aries

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Cyberlindnera jadinii D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+
-
 Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Rattus norvegicus D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r
1.1.1.44 6-phospho-D-gluconate + NADP+ enzyme specific for the oxidative pentose phosphate pathway Ovis aries D-ribulose 5-phosphate + CO2 + NADPH + H+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.44 7.7
-
 low substrate concentrations Ovis aries
1.1.1.44 9.1
-
-
 Ovis aries

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.44 5.5 9
-
 Ovis aries

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.44 9.1
-
 unstable at higher pH values Ovis aries