BRENDA - Enzyme Database

Biochemical and phylogenetic characterization of isocitrate dehydrogenase from a hyperthermophilic archaeon, Archaeoglobus fulgidus

Steen, I.H.; Lien.T.; Birkeland, N-K.; Arch. MIcrobiol. 168, 412-420 (1997)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.03
-
NADP+
-
Archaeoglobus fulgidus
1.1.1.42
0.118
-
D,L-isocitrate
-
Archaeoglobus fulgidus
1.1.1.42
5.4
-
NAD+
-
Archaeoglobus fulgidus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Archaeoglobus fulgidus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Archaeoglobus fulgidus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
42000
-
alpha2, 2 * 42000, SDS-PAGE
Archaeoglobus fulgidus
1.1.1.42
80000
-
gel filtration
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Archaeoglobus fulgidus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Archaeoglobus fulgidus
O29610
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
141
-
-
Archaeoglobus fulgidus
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.42
4°C, several months, under oxic conditions
Archaeoglobus fulgidus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286808
Archaeoglobus fulgidus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 42000, SDS-PAGE
Archaeoglobus fulgidus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
90
-
-
Archaeoglobus fulgidus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
90
-
half life of 22 min in 50 mM tricine-KOH buffer, pH 8
Archaeoglobus fulgidus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8.6
-
-
Archaeoglobus fulgidus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NAD+
-
Archaeoglobus fulgidus
1.1.1.42
NADP+
-
Archaeoglobus fulgidus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NAD+
-
Archaeoglobus fulgidus
1.1.1.42
NADP+
-
Archaeoglobus fulgidus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.03
-
NADP+
-
Archaeoglobus fulgidus
1.1.1.42
0.118
-
D,L-isocitrate
-
Archaeoglobus fulgidus
1.1.1.42
5.4
-
NAD+
-
Archaeoglobus fulgidus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Mg2+
absolute requirement for divalent cations
Archaeoglobus fulgidus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Archaeoglobus fulgidus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
42000
-
alpha2, 2 * 42000, SDS-PAGE
Archaeoglobus fulgidus
1.1.1.42
80000
-
gel filtration
Archaeoglobus fulgidus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Archaeoglobus fulgidus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Archaeoglobus fulgidus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.1.1.42
141
-
-
Archaeoglobus fulgidus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.42
4°C, several months, under oxic conditions
Archaeoglobus fulgidus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286808
Archaeoglobus fulgidus
2-oxoglutarate + NADPH + CO2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.42
dimer
alpha2, 2 * 42000, SDS-PAGE
Archaeoglobus fulgidus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
90
-
-
Archaeoglobus fulgidus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
90
-
half life of 22 min in 50 mM tricine-KOH buffer, pH 8
Archaeoglobus fulgidus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
8.6
-
-
Archaeoglobus fulgidus