BRENDA - Enzyme Database

Purification and properties of NADP+:isocitrate dehydrogenase from lactating bovine mammary gland

Farrell, H.M.; Arch. Biochem. Biophys. 204, 551-559 (1980)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
1.1.1.42
stabilization by dithiothreitol
Bos taurus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
glyoxylate
plus oxaloacetate
Bos taurus
1.1.1.42
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.011
-
NADP+
-
Bos taurus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Cd2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Co2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Zn2+
absolute requirement for divalent cations
Bos taurus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
55000
-
PAGE
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bos taurus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mammary gland
-
Bos taurus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286788
Bos taurus
2-oxoglutarate + NADPH + CO2
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
8.2
-
Bos taurus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6
9.5
-
Bos taurus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
6
-
-
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.42
NADP+
-
Bos taurus
General Stability (protein specific)
EC Number
General Stability
Organism
1.1.1.42
stabilization by dithiothreitol
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
glyoxylate
plus oxaloacetate
Bos taurus
1.1.1.42
p-chloromercuribenzoate
-
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.011
-
NADP+
-
Bos taurus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.42
Cd2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Co2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Mg2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Mn2+
absolute requirement for divalent cations
Bos taurus
1.1.1.42
Zn2+
absolute requirement for divalent cations
Bos taurus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
55000
-
PAGE
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Bos taurus
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.42
mammary gland
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286788
Bos taurus
2-oxoglutarate + NADPH + CO2
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.42
7.2
8.2
-
Bos taurus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.42
6
9.5
-
Bos taurus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
6
-
-
Bos taurus