BRENDA - Enzyme Database

Purification and properties of isocitrate dehydrogenase (NADP) from Thermus aquaticus YT-1, Bacillus subtilis-168 and Chlamydomonas reinhardti-Y-2

Ramaley, R.F.; Hudock, M.O.; Biochim. Biophys. Acta 315, 22-36 (1973)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
glyoxylate
plus oxaloacetate
Bacillus subtilis
1.1.1.42
glyoxylate
plus oxaloacetate
Chlamydomonas reinhardtii
1.1.1.42
glyoxylate
plus oxaloacetate
Thermus aquaticus
1.1.1.42
Oxalomalate
-
Chlamydomonas reinhardtii
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0091
-
D,L-isocitrate
-
Bacillus subtilis
1.1.1.42
0.0097
-
D,L-isocitrate
25°C assay temperature
Thermus aquaticus
1.1.1.42
0.01
-
NADP+
-
Chlamydomonas reinhardtii
1.1.1.42
0.0125
-
D,L-isocitrate
-
Chlamydomonas reinhardtii
1.1.1.42
0.0165
-
NADP+
25°C assay temperature
Thermus aquaticus
1.1.1.42
0.0181
-
D,L-isocitrate
70°C assay temperature
Thermus aquaticus
1.1.1.42
0.0274
-
NADP+
-
Bacillus subtilis
1.1.1.42
0.031
-
NADP+
70°C assay temperature
Thermus aquaticus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
70000
gel filtration
Thermus aquaticus
1.1.1.42
75000
80000
gel filtration
Bacillus subtilis
1.1.1.42
80000
-
gel filtration
Azotobacter vinelandii
1.1.1.42
80000
-
gel filtration
Escherichia coli
1.1.1.42
87000
92600
gel filtration
Geobacillus stearothermophilus
1.1.1.42
90000
100000
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermus aquaticus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Chlamydomonas reinhardtii
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Bacillus subtilis
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Azotobacter vinelandii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.42
Azotobacter vinelandii
-
-
-
1.1.1.42
Bacillus subtilis
-
-
-
1.1.1.42
Chlamydomonas reinhardtii
-
-
-
1.1.1.42
Escherichia coli
-
-
-
1.1.1.42
Geobacillus stearothermophilus
-
-
-
1.1.1.42
Thermus aquaticus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.42
-
Bacillus subtilis
1.1.1.42
-
Thermus aquaticus
1.1.1.42
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286779
Thermus aquaticus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Chlamydomonas reinhardtii
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Bacillus subtilis
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Geobacillus stearothermophilus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Azotobacter vinelandii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
-
assay condition
Bacillus subtilis
1.1.1.42
25
-
assay condition
Chlamydomonas reinhardtii
1.1.1.42
25
-
assay condition
Geobacillus stearothermophilus
1.1.1.42
25
-
assay condition
Thermus aquaticus
1.1.1.42
37
-
-
Bacillus subtilis
1.1.1.42
70
-
-
Thermus aquaticus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
30
-
50% loss of activity in 1 h
Chlamydomonas reinhardtii
1.1.1.42
54
-
50% loss of activity in 1 h
Bacillus subtilis
1.1.1.42
88
-
50% loss of activity in 1 h
Thermus aquaticus
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
6
9
-
Thermus aquaticus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.42
glyoxylate
plus oxaloacetate
Bacillus subtilis
1.1.1.42
glyoxylate
plus oxaloacetate
Chlamydomonas reinhardtii
1.1.1.42
glyoxylate
plus oxaloacetate
Thermus aquaticus
1.1.1.42
Oxalomalate
-
Chlamydomonas reinhardtii
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.42
0.0091
-
D,L-isocitrate
-
Bacillus subtilis
1.1.1.42
0.0097
-
D,L-isocitrate
25°C assay temperature
Thermus aquaticus
1.1.1.42
0.01
-
NADP+
-
Chlamydomonas reinhardtii
1.1.1.42
0.0125
-
D,L-isocitrate
-
Chlamydomonas reinhardtii
1.1.1.42
0.0165
-
NADP+
25°C assay temperature
Thermus aquaticus
1.1.1.42
0.0181
-
D,L-isocitrate
70°C assay temperature
Thermus aquaticus
1.1.1.42
0.0274
-
NADP+
-
Bacillus subtilis
1.1.1.42
0.031
-
NADP+
70°C assay temperature
Thermus aquaticus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.42
60000
70000
gel filtration
Thermus aquaticus
1.1.1.42
75000
80000
gel filtration
Bacillus subtilis
1.1.1.42
80000
-
gel filtration
Azotobacter vinelandii
1.1.1.42
80000
-
gel filtration
Escherichia coli
1.1.1.42
87000
92600
gel filtration
Geobacillus stearothermophilus
1.1.1.42
90000
100000
gel filtration
Chlamydomonas reinhardtii
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.42
isocitrate + NADP+
Thermus aquaticus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Chlamydomonas reinhardtii
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Bacillus subtilis
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Escherichia coli
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Geobacillus stearothermophilus
-
2-oxoglutarate + NADPH + CO2
-
-
?
1.1.1.42
isocitrate + NADP+
Azotobacter vinelandii
-
2-oxoglutarate + NADPH + CO2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.42
-
Bacillus subtilis
1.1.1.42
-
Thermus aquaticus
1.1.1.42
-
Chlamydomonas reinhardtii
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.42
isocitrate + NADP+
-
286779
Thermus aquaticus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Chlamydomonas reinhardtii
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Bacillus subtilis
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Escherichia coli
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Geobacillus stearothermophilus
2-oxoglutarate + NADPH + CO2
-
-
-
?
1.1.1.42
isocitrate + NADP+
-
286779
Azotobacter vinelandii
2-oxoglutarate + NADPH + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.42
25
-
assay condition
Bacillus subtilis
1.1.1.42
25
-
assay condition
Chlamydomonas reinhardtii
1.1.1.42
25
-
assay condition
Geobacillus stearothermophilus
1.1.1.42
25
-
assay condition
Thermus aquaticus
1.1.1.42
37
-
-
Bacillus subtilis
1.1.1.42
70
-
-
Thermus aquaticus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.42
30
-
50% loss of activity in 1 h
Chlamydomonas reinhardtii
1.1.1.42
54
-
50% loss of activity in 1 h
Bacillus subtilis
1.1.1.42
88
-
50% loss of activity in 1 h
Thermus aquaticus
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.42
6
9
-
Thermus aquaticus