BRENDA - Enzyme Database

Determinants of cofactor specificity in isocitrate dehydrogenase: structure of an engineered NADP+ to NAD+ specificity-reversal mutant

Hurley, J.H.; Chen, R.; Dean, A.M.; Biochemistry 35, 5670-5678 (1996)

Data extracted from this reference:

Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.41
crystallization in artificial mother liquor supplemented with 100 mM NAD+
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
C201M/C332Y/K344D/Y345I/V351A/Y391K/R395S
converts the cofactor specificity from 7000-fold preference of NADP+ to a 200-fold preference of NAD+
Escherichia coli
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.017
-
NADP+
wild type
Escherichia coli
1.1.1.41
0.099
-
NAD+
7-fold mutant
Escherichia coli
1.1.1.41
4.7
-
NAD+
wild type
Escherichia coli
1.1.1.41
5.8
-
NADP+
7-fold mutant
Escherichia coli
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Escherichia coli
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Escherichia coli
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286777
Escherichia coli
2-oxoglutarate + CO2 + NADH
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Escherichia coli
1.1.1.41
NADP+
-
Escherichia coli
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Escherichia coli
1.1.1.41
NADP+
-
Escherichia coli
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.41
crystallization in artificial mother liquor supplemented with 100 mM NAD+
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.1.41
C201M/C332Y/K344D/Y345I/V351A/Y391K/R395S
converts the cofactor specificity from 7000-fold preference of NADP+ to a 200-fold preference of NAD+
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.017
-
NADP+
wild type
Escherichia coli
1.1.1.41
0.099
-
NAD+
7-fold mutant
Escherichia coli
1.1.1.41
4.7
-
NAD+
wild type
Escherichia coli
1.1.1.41
5.8
-
NADP+
7-fold mutant
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Escherichia coli
-
2-oxoglutarate + CO2 + NADH
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286777
Escherichia coli
2-oxoglutarate + CO2 + NADH
-
-
-
?