BRENDA - Enzyme Database

Partial purification and characteristics of membrane-associated NAD+-dependent isocitrate dehydrogenase activity from etiolated pea mitochondria

McIntosh, C.A.; Plant Sci. 129, 9-20 (1997)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
NADH
competitive inhibitor
Pisum sativum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.33
-
NAD+
membrane-associated ICDH
Pisum sativum
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Pisum sativum
1.1.1.41
Mn2+
requires divalent cations
Pisum sativum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
320000
-
gel filtration
Pisum sativum
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Pisum sativum
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
-
Pisum sativum
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.41
4C, glycerol 20%, 35-40% of original activity after 96 h, membrane-associated ICDH
Pisum sativum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286774
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
-
Pisum sativum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Pisum sativum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Pisum sativum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.41
NADH
competitive inhibitor
Pisum sativum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.41
0.33
-
NAD+
membrane-associated ICDH
Pisum sativum
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.1.1.41
mitochondrion
-
Pisum sativum
5739
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Mg2+
-
Pisum sativum
1.1.1.41
Mn2+
requires divalent cations
Pisum sativum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.41
320000
-
gel filtration
Pisum sativum
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Pisum sativum
-
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
-
Pisum sativum
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.41
4C, glycerol 20%, 35-40% of original activity after 96 h, membrane-associated ICDH
Pisum sativum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286774
Pisum sativum
2-oxoglutarate + CO2 + NADH
-
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.41
7.5
-
-
Pisum sativum