BRENDA - Enzyme Database

Role of manganous ion in the kinetics of pig-heart NAD-specific isocitrate dehydrogenase

Cohen, P.F.; Colman, R.F.; Eur. J. Biochem. 47, 35-45 (1974)

Data extracted from this reference:

Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Co2+
less effective than Mg2+ or Mn2+
Sus scrofa
1.1.1.41
Mg2+
required, active substrate is Mg-DL-isocitrate chelate
Sus scrofa
1.1.1.41
Mn2+
requires divalent cations
Sus scrofa
1.1.1.41
Zn2+
less effective than Mn2+ or Mg2+
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Sus scrofa
-
2-oxoglutarate + CO2 + NADH
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.41
Sus scrofa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.41
-
Sus scrofa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
heart
-
Sus scrofa
-
Storage Stability
EC Number
Storage Stability
Organism
1.1.1.41
-90C, no loss of activity after 6 weeks
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286751
Sus scrofa
2-oxoglutarate + CO2 + NADH
-
-
-
?
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6
8
-
Sus scrofa
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.41
NAD+
-
Sus scrofa
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.41
Co2+
less effective than Mg2+ or Mn2+
Sus scrofa
1.1.1.41
Mg2+
required, active substrate is Mg-DL-isocitrate chelate
Sus scrofa
1.1.1.41
Mn2+
requires divalent cations
Sus scrofa
1.1.1.41
Zn2+
less effective than Mn2+ or Mg2+
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.41
isocitrate + NAD+
Sus scrofa
-
2-oxoglutarate + CO2 + NADH
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.41
-
Sus scrofa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.41
heart
-
Sus scrofa
-
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.1.1.41
-90C, no loss of activity after 6 weeks
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.41
isocitrate + NAD+
-
286751
Sus scrofa
2-oxoglutarate + CO2 + NADH
-
-
-
?
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.41
6
8
-
Sus scrofa