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Literature summary extracted from

  • Farias de Aragao, M.E.; Jolivet, Y.; Fernandes de Melo, D.; da Guia Silva Lima, M.; Dizengremel, P.
    Purification and partial characterization of NAD-dependent malic enzyme isolated from Vigna unguiculata hypocotyls (1996), Plant Physiol. Biochem., 34, 363-368.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.39 additional information
-
 additional information
-
 Vigna unguiculata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.39 Mg2+ preferably used as metal cofactor Vigna unguiculata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.39 61000
-
 1 * 61000 + 1 * 64000, SDS-PAGE Vigna unguiculata
1.1.1.39 64000
-
 1 * 61000 + 1 * 64000, SDS-PAGE Vigna unguiculata
1.1.1.39 120000
-
 non-denaturing PAGE Vigna unguiculata

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.39 Vigna unguiculata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.39
-
 Vigna unguiculata

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.39 (S)-malate + NAD+ = pyruvate + CO2 + NADH + H+ sequential mechanism with each substrate bound randomly Vigna unguiculata
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.39 hypocotyl etiolated Vigna unguiculata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.39 additional information
-
-
 Vigna unguiculata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.39 malate + NAD+
-
 Vigna unguiculata pyruvate + CO2 + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.39 dimer 1 * 61000 + 1 * 64000, SDS-PAGE Vigna unguiculata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.39 6.8
-
-
 Vigna unguiculata

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.39 NAD+ cofactor Vigna unguiculata