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Literature summary extracted from

  • Ziegler, I.
    Malate dehydrogenase of spinach: Substrate kinetics of different forms (1974), Phytochemistry, 13, 2403-2410.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.1.1.37 both Mg2+ and NADH stabilizes the 127000 Da MW form Spinacia oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.37 mitochondrion
-
 Neurospora crassa 5739
-
1.1.1.37 mitochondrion
-
 Spinacia oleracea 5739
-
1.1.1.37 mitochondrion
-
 Lemna minor 5739
-
1.1.1.37 peroxisome
-
 Spinacia oleracea 5777
-
1.1.1.37 peroxisome
-
 Lemna minor 5777
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.37 32000
-
-
 Spinacia oleracea
1.1.1.37 35000
-
 K+ and/or dithioerythritol treated Spinacia oleracea
1.1.1.37 39000
-
-
 Spinacia oleracea
1.1.1.37 64000
-
 repeatedly frozen and thawed, most stable unit, gel filtration Spinacia oleracea
1.1.1.37 70000
-
 various isoenzymes Lemna minor
1.1.1.37 127000
-
 gel filtration Spinacia oleracea
1.1.1.37 140000
-
 various isoenzymes Lemna minor
1.1.1.37 180000
-
 K+ and/or dithioerythritol treated Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.37 malate + NAD+ Neurospora crassa
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Spinacia oleracea
-
 oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+ Lemna minor
-
 oxaloacetate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.37 Lemna minor
-
-
-
1.1.1.37 Neurospora crassa
-
-
-
1.1.1.37 Spinacia oleracea
-
 spinach
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.37 leaf
-
 Spinacia oleracea
-
1.1.1.37 leaf
-
 Lemna minor
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.37 (S)-malate + NAD+
-
 Neurospora crassa oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 (S)-malate + NAD+
-
 Lemna minor oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Neurospora crassa oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Spinacia oleracea oxaloacetate + NADH + H+
-
 r
1.1.1.37 malate + NAD+
-
 Lemna minor oxaloacetate + NADH + H+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Neurospora crassa L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Spinacia oleracea L-malate + NAD+
-
 r
1.1.1.37 oxaloacetate + NADH
-
 Lemna minor L-malate + NAD+
-
 r