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Literature summary extracted from

  • Shimakata, T.; Fujita, Y.; Kusaka, T.
    Purification and characterization of 3-hydroxyacyl-CoA dehydrogenase of Mycobacterium smegmatis (1979), J. Biochem., 86, 1191-1198.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.35 p-chloromercuriphenyl sulfonic acid 95% inhibition at 0.1 mM Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.35 0.033
-
 NADH
-
 Mycolicibacterium smegmatis
1.1.1.35 0.036
-
 acetoacetyl-CoA
-
 Mycolicibacterium smegmatis
1.1.1.35 0.083
-
 NAD+
-
 Mycolicibacterium smegmatis
1.1.1.35 1.19
-
 acetoacetyl-pantetheine
-
 Mycolicibacterium smegmatis
1.1.1.35 44.4
-
 acetoacetyl-N-acetyl-cysteamine
-
 Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.35 50300
-
 gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+ Mycolicibacterium smegmatis
-
 acetoacetyl-CoA + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.35 Mycolicibacterium smegmatis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.35 ammonium sulfate, Sephadex G-150, hydroxyapatite, NAD-Sepharose 4B Mycolicibacterium smegmatis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.35 41.2
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NAD+
-
 Mycolicibacterium smegmatis acetoacetyl-CoA + NADH
-
 r
1.1.1.35 (S)-3-hydroxybutyryl-CoA + NADP+
-
 Mycolicibacterium smegmatis acetoacetyl-CoA + NADPH
-
 ?
1.1.1.35 acetoacetyl-N-acetylcysteamine + NADH
-
 Mycolicibacterium smegmatis (S)-3-hydroxybutyryl-N-acetylcysteamine + NAD+
-
 ?
1.1.1.35 acetoacetyl-pantetheine + NADH
-
 Mycolicibacterium smegmatis (S)-3-hydroxybutyryl-pantetheine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.35 6
-
 reduction of acetoacetyl-CoA Mycolicibacterium smegmatis
1.1.1.35 9
-
 oxidation of (S)-3-hydroxybutyryl-CoA Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.35 NAD+
-
 Mycolicibacterium smegmatis
1.1.1.35 NADP+
-
 Mycolicibacterium smegmatis