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Literature summary extracted from

  • Dhariwal, K.R.; Venkitasubramanian, T.A.
    Purification and properties of beta-hydroxybutyrate dehydrogenase from Mycobacterium phlei ATCC354 (1978), J. Gen. Microbiol., 104, 123-126.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Mycolicibacterium phlei

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.30 HgCl2 enzyme extremely sensitive, NADH and Ca2+ protect Mycolicibacterium phlei
1.1.1.30 p-chloromercuribenzoate enzyme extremely sensitive, NADH and Ca2+ protect Mycolicibacterium phlei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.66
-
 NAD+
-
 Mycolicibacterium phlei
1.1.1.30 7.4
-
 (R)-3-hydroxybutanoate
-
 Mycolicibacterium phlei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Mycolicibacterium phlei
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Mycolicibacterium phlei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 ammonium sulfate precipitation, DEAE-cellulose Mycolicibacterium phlei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 14
-
-
 Mycolicibacterium phlei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Mycolicibacterium phlei acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Mycolicibacterium phlei acetoacetate + NADH + H+
-
 r

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.30 37
-
 loss of 70% activity after 15 min, 0.24 mg protein/ml, Ca2+ and Mn2+ stabilize enzyme Mycolicibacterium phlei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 6.4 7 reduction of acetoacetate Mycolicibacterium phlei
1.1.1.30 8.4
-
 oxidation of beta-hydroxybutyrate Mycolicibacterium phlei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.30 7.6 9 oxidation of beta-hydroxybutyrate Mycolicibacterium phlei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Mycolicibacterium phlei