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Literature summary extracted from

  • Green, D.; Marks, a.R.; Fleischer, S.; McIntyre, J.O.
    Wild type and mutant human heart (R)-3-hydroxybutyrate dehydrogenase expressed in insect cells (1996), Biochemistry, 35, 8158-8165.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 phosphatidylcholine
-
 Homo sapiens
1.1.1.30 phosphatidylcholine
-
 Bos taurus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.30 mature form, expressed in Spodoptera frugiperda, SF9 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.30 C242S PCR derived cDNA clone Homo sapiens
1.1.1.30 M92V PCR derived cDNA clone Homo sapiens
1.1.1.30 S24T PCR derived cDNA clone Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 0.53
-
 NAD+ enzyme expressed in SF9 cells Homo sapiens
1.1.1.30 0.59
-
 (R)-3-hydroxybutanoate submitochondrial vesicles Bos taurus
1.1.1.30 0.71
-
 (R)-3-hydroxybutanoate enzyme expressed in SF9 cells Homo sapiens
1.1.1.30 0.8
-
 NAD+ enzyme expressed in SF9 cells, C242S mutant Homo sapiens
1.1.1.30 0.95
-
 NAD+ submitochondrial vesicles Bos taurus
1.1.1.30 10
-
 (R)-3-hydroxybutanoate enzyme expressed in SF9 cells, C242S mutant, C242 important for substrate binding Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.30 cytoplasm
-
 Homo sapiens 5737
-
1.1.1.30 cytoplasm
-
 Bos taurus 5737
-
1.1.1.30 mitochondrion located on the matrix face of the inner membrane Homo sapiens 5739
-
1.1.1.30 mitochondrion located on the matrix face of the inner membrane Bos taurus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Homo sapiens
-
 acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+ Bos taurus
-
 acetoacetate + NADH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Bos taurus
-
-
-
1.1.1.30 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.30 heart
-
 Homo sapiens
-
1.1.1.30 heart
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 0.17
-
 solubilized apo-3-hydroxybutyrate dehydrogenase, reconstituted with phosphatidylcholine/phosphatidylethanolamine/diphosphatidylglycerol, 5/4/1 Bos taurus
1.1.1.30 0.22
-
 apo-3-hydroxybutyrate dehydrogenase, reconstituted with phosphatidylcholine/phosphatidylethanolamine/diphosphatidylglycerol, 5/4/1 Homo sapiens
1.1.1.30 0.5
-
 enzyme expressed in SF9 cells, S24T mutatant Homo sapiens
1.1.1.30 0.52
-
 submitochondrial vesicles, membrane bound enzyme Bos taurus
1.1.1.30 0.6
-
 enzyme expressed in SF9 cells, M92V mutatant Homo sapiens
1.1.1.30 0.63
-
 enzyme expressed in SF9 cells, membrane bound Homo sapiens
1.1.1.30 0.8
-
 enzyme expressed in SF9 cells, C242S mutatant Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Homo sapiens acetoacetate + NADH
-
 r
1.1.1.30 (R)-3-hydroxybutanoate + NAD+
-
 Bos taurus acetoacetate + NADH
-
 r

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Homo sapiens
1.1.1.30 NAD+
-
 Bos taurus