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Literature summary extracted from

  • Long, G.L.
    D-Lactate dehydrogenase from the horseshoe crab (1975), Methods Enzymol., 41B, 313-323.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.28 2-mercaptoethanol and EDTA stabilize Limulus polyphemus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.28 iodoacetamide
-
 Limulus polyphemus
1.1.1.28 iodoacetate
-
 Limulus polyphemus
1.1.1.28 oxamate
-
 Limulus polyphemus
1.1.1.28 p-hydroxymercuribenzoate
-
 Limulus polyphemus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.28 0.00021
-
 NADH
-
 Limulus polyphemus
1.1.1.28 0.00044
-
 NAD+
-
 Limulus polyphemus
1.1.1.28 0.07
-
 pyruvate
-
 Limulus polyphemus
1.1.1.28 3.8
-
 (R)-lactate
-
 Limulus polyphemus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.28 35000
-
 2 * 35000, gel filtration Limulus polyphemus
1.1.1.28 70000 80000 gel filtration, equilibrium sedimentation Limulus polyphemus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+ Limulus polyphemus
-
 pyruvate + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.28 Limulus polyphemus
-
 horseshoe crab
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.28
-
 Limulus polyphemus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.28 skeletal muscle
-
 Limulus polyphemus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.28 (R)-lactate + NAD+
-
 Limulus polyphemus pyruvate + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.28 dimer 2 * 35000, gel filtration Limulus polyphemus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.28 50
-
 3 min, 5% loss of activity Limulus polyphemus
1.1.1.28 60
-
 3 min, 90% loss of activity Limulus polyphemus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.28 633
-
 NADH
-
 Limulus polyphemus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.28 NAD+
-
 Limulus polyphemus
1.1.1.28 NADH
-
 Limulus polyphemus