BRENDA - Enzyme Database

L-Lactate dehydrogenase from leaves of higher plants. Kinetics and regulation of the enzyme from lettuce (Lactuca sativa L)

Betsche, T.; Biochem. J. 195, 615-622 (1981)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.27
3-phosphoglycerate
-
Lactuca sativa
1.1.1.27
ADP
-
Lactuca sativa
1.1.1.27
Ala
-
Lactuca sativa
1.1.1.27
AMP
-
Lactuca sativa
1.1.1.27
ATP
competitive with respect to NADH at pH 7.0 and at pH 6.2
Lactuca sativa
1.1.1.27
citrate
-
Lactuca sativa
1.1.1.27
fructose 1,6-diphosphate
-
Lactuca sativa
1.1.1.27
glucose 6-phosphate
-
Lactuca sativa
1.1.1.27
Gly
-
Lactuca sativa
1.1.1.27
isocitrate
-
Lactuca sativa
1.1.1.27
oxalate
-
Lactuca sativa
1.1.1.27
Ser
-
Lactuca sativa
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.27
0.35
-
pyruvate
pH 6.2
Lactuca sativa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.27
additional information
Lactuca sativa
the enzyme is a component of the system regulating the cellular pH and/or controlling the concentration of reducing equivalents in the cytoplasm of leaf cells
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.27
Lactuca sativa
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.27
-
Lactuca sativa
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.27
leaf
-
Lactuca sativa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.27
additional information
-
-
Lactuca sativa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.27
(S)-lactate + NAD+
-
286447
Lactuca sativa
pyruvate + NADH + H+
-
286447
Lactuca sativa
r
1.1.1.27
glyoxylate + NADH
-
286447
Lactuca sativa
glycolate + NAD+
-
-
-
?
1.1.1.27
hydroxypyruvate + NADPH + H+
-
286447
Lactuca sativa
2,3-dihydroxypropanoate + NADP+
-
-
-
?
1.1.1.27
additional information
the enzyme is a component of the system regulating the cellular pH and/or controlling the concentration of reducing equivalents in the cytoplasm of leaf cells
286447
Lactuca sativa
?
-
-
-
-
1.1.1.27
pyruvate + NADH + H+
-
286447
Lactuca sativa
(S)-lactate + NAD+
-
286447
Lactuca sativa
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.27
NAD+
coenzyme
Lactuca sativa
1.1.1.27
NADH
coenzyme
Lactuca sativa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.27
NAD+
coenzyme
Lactuca sativa
1.1.1.27
NADH
coenzyme
Lactuca sativa
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.27
3-phosphoglycerate
-
Lactuca sativa
1.1.1.27
ADP
-
Lactuca sativa
1.1.1.27
Ala
-
Lactuca sativa
1.1.1.27
AMP
-
Lactuca sativa
1.1.1.27
ATP
competitive with respect to NADH at pH 7.0 and at pH 6.2
Lactuca sativa
1.1.1.27
citrate
-
Lactuca sativa
1.1.1.27
fructose 1,6-diphosphate
-
Lactuca sativa
1.1.1.27
glucose 6-phosphate
-
Lactuca sativa
1.1.1.27
Gly
-
Lactuca sativa
1.1.1.27
isocitrate
-
Lactuca sativa
1.1.1.27
oxalate
-
Lactuca sativa
1.1.1.27
Ser
-
Lactuca sativa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.1.27
0.35
-
pyruvate
pH 6.2
Lactuca sativa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.1.27
additional information
Lactuca sativa
the enzyme is a component of the system regulating the cellular pH and/or controlling the concentration of reducing equivalents in the cytoplasm of leaf cells
?
-
-
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.27
-
Lactuca sativa
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.1.1.27
leaf
-
Lactuca sativa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.27
additional information
-
-
Lactuca sativa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.27
(S)-lactate + NAD+
-
286447
Lactuca sativa
pyruvate + NADH + H+
-
286447
Lactuca sativa
r
1.1.1.27
glyoxylate + NADH
-
286447
Lactuca sativa
glycolate + NAD+
-
-
-
?
1.1.1.27
hydroxypyruvate + NADPH + H+
-
286447
Lactuca sativa
2,3-dihydroxypropanoate + NADP+
-
-
-
?
1.1.1.27
additional information
the enzyme is a component of the system regulating the cellular pH and/or controlling the concentration of reducing equivalents in the cytoplasm of leaf cells
286447
Lactuca sativa
?
-
-
-
-
1.1.1.27
pyruvate + NADH + H+
-
286447
Lactuca sativa
(S)-lactate + NAD+
-
286447
Lactuca sativa
r