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Literature summary extracted from

  • Betsche, T.; Schaller, D.; Melkonian, M.
    Identification and characteriaztion of glycolate oxidase and related enzymes from the endocyanotic alga Cyanophora paradoxa and from pea leaves (1992), Plant Physiol., 98, 887-893.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.26 Acetohydroxamate inhibits only the cytosolic enzyme type with preference for glyoxylate as substrate Pisum sativum
1.1.1.26 MOPS buffer 50 mM at acidic and alkaline pH Cyanophora paradoxa
1.1.1.26 oxalate inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate Pisum sativum
1.1.1.26 Tartronate inhibits only the cytosolic enzyme type with preference for hydroxypyruvate as substrate Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.26 chloroplast utilizes NADH and NADPH equally Pisum sativum 9507
-
1.1.1.26 cyanelle
-
 Cyanophora paradoxa 9842
-
1.1.1.26 cytosol NADP+/NADPH preferring type Pisum sativum 5829
-
1.1.1.26 peroxisome NAD+/NADH preferring type Pisum sativum 5777
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.26 glycolate + NAD+ Cyanophora paradoxa
-
 glyoxylate + NADH
-
 r
1.1.1.26 glycolate + NAD+ Pisum sativum
-
 glyoxylate + NADH
-
 r
1.1.1.26 glyoxylate + NADH Cyanophora paradoxa
-
 glycolate + NAD+
-
 r
1.1.1.26 glyoxylate + NADH Pisum sativum
-
 glycolate + NAD+
-
 r
1.1.1.26 hydroxypyruvate + NADH Cyanophora paradoxa
-
 D-glycerate + NAD+
-
 r
1.1.1.26 hydroxypyruvate + NADH Pisum sativum
-
 D-glycerate + NAD+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.26 Cyanophora paradoxa
-
-
-
1.1.1.26 Pisum sativum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.26
-
 Cyanophora paradoxa
1.1.1.26
-
 Pisum sativum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.26 glycolate + NAD+ = glyoxylate + NADH + H+ can convert L-lactate Pisum sativum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.26 leaf
-
 Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.26 0.032
-
 0.5 mM NADPH Pisum sativum
1.1.1.26 0.044
-
 75 mM NADH Cyanophora paradoxa
1.1.1.26 0.077
-
 0.5 mM NADH Pisum sativum
1.1.1.26 0.095
-
 0.5 mM NADPH Cyanophora paradoxa
1.1.1.26 0.096
-
 0.5 mM NADH Cyanophora paradoxa
1.1.1.26 0.11
-
 0.5 mM NADPH Cyanophora paradoxa
1.1.1.26 0.134
-
 0.5 mM NADH Cyanophora paradoxa
1.1.1.26 0.16
-
 0.5 mM NADPH Pisum sativum
1.1.1.26 0.205
-
 NADH Pisum sativum
1.1.1.26 0.738
-
 0.5 mM NADH Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.26 glycolate + NAD+
-
 Cyanophora paradoxa glyoxylate + NADH
-
 r
1.1.1.26 glycolate + NAD+
-
 Pisum sativum glyoxylate + NADH
-
 r
1.1.1.26 glyoxylate + NADH
-
 Cyanophora paradoxa glycolate + NAD+
-
 r
1.1.1.26 glyoxylate + NADH
-
 Pisum sativum glycolate + NAD+
-
 r
1.1.1.26 glyoxylate + NADH equilibrium is very far in direction of glycolate formation Cyanophora paradoxa glycolate + NAD+
-
 r
1.1.1.26 glyoxylate + NADH equilibrium is very far in direction of glycolate formation Pisum sativum glycolate + NAD+
-
 r
1.1.1.26 hydroxypyruvate + NADH
-
 Cyanophora paradoxa D-glycerate + NAD+
-
 r
1.1.1.26 hydroxypyruvate + NADH
-
 Pisum sativum D-glycerate + NAD+
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.26 7.9
-
 Tes/K+, substrate glyoxylate Cyanophora paradoxa
1.1.1.26 8.2
-
 Tris/HCl, substrate glyoxylate Cyanophora paradoxa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.26 6.5 11
-
 Cyanophora paradoxa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.26 NAD+
-
 Pisum sativum
1.1.1.26 NAD+ can also utilize NADP+ or O2 (reduction to H2O2) as cofactors Cyanophora paradoxa
1.1.1.26 NADH
-
 Pisum sativum
1.1.1.26 NADH can also utilize NADPH or O2 (reduction to H2O2) as cofactors Cyanophora paradoxa