Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Nagai, A.; Suzuku, K.; Ward, E.; Moyer, M.; Hashimoto, M.; Mano, J.; Ohta, D.; Scheidegger, A.
    Overexpression of plant histidinol dehydrogenase using baculovirus expression vector system (1992), Arch. Biochem. Biophys., 295, 235-239.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.23 expressed in Spodoptera frugiperda, SF9 cells Brassica oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.23 Ca2+
-
 Brassica oleracea
1.1.1.23 Cu2+
-
 Brassica oleracea
1.1.1.23 Mg2+
-
 Brassica oleracea
1.1.1.23 Zn2+
-
 Brassica oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.23 0.01
-
 L-histidinol recombinant enzyme Brassica oleracea
1.1.1.23 0.012
-
 L-histidinol
-
 Brassica oleracea
1.1.1.23 0.042
-
 NAD+
-
 Brassica oleracea
1.1.1.23 0.05
-
 NAD+ recombinant enzyme Brassica oleracea

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.23 Mn2+
-
 Brassica oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.23 52000
-
 alpha,alpha, 2 * 52000, SDS-PAGE Brassica oleracea
1.1.1.23 100000
-
 recombinant enzyme expressed in SF9 cells, native PAGE Brassica oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O Brassica oleracea reaction intermediate L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O Brassica oleracea
-
 L-histidine + 2 NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.23 Brassica oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.23 enzyme expressed in SF9 cells, cell homogenate, Mono Q FPLC Brassica oleracea

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.23 10
-
 recombinant enzym Brassica oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.23 L-histidinal + NAD+ + H2O
-
 Brassica oleracea L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinal + NAD+ + H2O reaction intermediate Brassica oleracea L-histidine + NADH + H+
-
 ?
1.1.1.23 L-histidinol + 2 NAD+ + H2O
-
 Brassica oleracea L-histidine + 2 NADH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.23 homodimer alpha,alpha, 2 * 52000, SDS-PAGE Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.23 9.2
-
 recombinant enzyme Brassica oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.23 NAD+
-
 Brassica oleracea