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Literature summary extracted from

  • Wermuth, B.; Burgisser, H.; Bohren, K.; von Wartburg, J.P.
    Purification and characterization of human-brain aldose reductase (1982), Eur. J. Biochem., 127, 279-284.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.21 SO42- stimulation Homo sapiens

General Stability

EC Number General Stability Organism
1.1.1.21 freezing and thawing at temperatures above 50°C results in loss of activity Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.21 iodoacetate
-
 Homo sapiens
1.1.1.21 p-hydroxymercuribenzoate
-
 Homo sapiens
1.1.1.21 Phenobarbitone
-
 Homo sapiens
1.1.1.21 quercetin
-
 Homo sapiens
1.1.1.21 rutin
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.21 0.001
-
 20alpha-Isocorticosterone and 20alpha-isocortisol, values below Homo sapiens
1.1.1.21 0.002
-
 4-nitrobenzaldehyde
-
 Homo sapiens
1.1.1.21 0.003
-
 4-nitrobenzaldehyde 4-hydroxyphenylglycolaldehyde Homo sapiens
1.1.1.21 0.01
-
 4-hydroxyphenylacetaldehyde
-
 Homo sapiens
1.1.1.21 0.016
-
 Indole-3-acetaldehyde
-
 Homo sapiens
1.1.1.21 0.018
-
 L-glyceraldehyde
-
 Homo sapiens
1.1.1.21 0.025
-
 DL-glyceraldehyde
-
 Homo sapiens
1.1.1.21 0.035
-
 D-glyceraldehyde
-
 Homo sapiens
1.1.1.21 5
-
 D-glucuronate
-
 Homo sapiens
1.1.1.21 5.1
-
 D-xylose
-
 Homo sapiens
1.1.1.21 40
-
 D-galactose
-
 Homo sapiens
1.1.1.21 95
-
 D-glucose
-
 Homo sapiens
1.1.1.21 100
-
 L-Xylose
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.21 38000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.21 aldose + NAD(P)H Homo sapiens
-
 alditol + NAD(P)+
-
 r
1.1.1.21 additional information Homo sapiens enzyme involved in metabolism of corticosteroids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.21 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.21
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.21 brain
-
 Homo sapiens
-

Storage Stability

EC Number Storage Stability Organism
1.1.1.21 4°C, several weeks Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.21 20-isocortisol + NAD(P)H
-
 Homo sapiens ?
-
 ?
1.1.1.21 20alpha-isocorticosterone + NAD(P)H
-
 Homo sapiens ?
-
 ?
1.1.1.21 4-hydroxyphenylacetaldehyde + NAD(P)H
-
 Homo sapiens 4-hydroxyphenylethyl alcohol + NAD(P)+
-
 ?
1.1.1.21 4-hydroxyphenylglycolaldehyde + NAD(P)H
-
 Homo sapiens ?
-
 ?
1.1.1.21 4-nitrobenzaldehyde + NAD(P)H
-
 Homo sapiens 4-nitrobenzyl alcohol + NAD(P)+
-
 ?
1.1.1.21 alditol + NAD(P)+
-
 Homo sapiens aldose + NAD(P)H
-
 r
1.1.1.21 aldose + NAD(P)H
-
 Homo sapiens alditol + NAD(P)+
-
 r
1.1.1.21 D-galactose + NAD(P)H
-
 Homo sapiens dulcitol + NAD(P)+
-
 r
1.1.1.21 D-glucose + NAD(P)H
-
 Homo sapiens sorbitol + NAD(P)+
-
 r
1.1.1.21 D-glucuronate + NAD(P)H
-
 Homo sapiens ?
-
 r
1.1.1.21 D-glyceraldehyde + NAD(P)H
-
 Homo sapiens glycerol + NAD(P)+
-
 ?
1.1.1.21 D-xylose + NAD(P)H
-
 Homo sapiens xylitol + NAD(P)+
-
 r
1.1.1.21 DL-glyceraldehyde + NAD(P)H
-
 Homo sapiens glycerol + NAD(P)+
-
 r
1.1.1.21 indole-3-acetaldehyde + NAD(P)H
-
 Homo sapiens indole-3-ethanol + NAD(P)+
-
 ?
1.1.1.21 L-glyceraldehyde + NADPH + H+
-
 Homo sapiens glycerol + NADP+
-
 r
1.1.1.21 L-xylose + NAD(P)H
-
 Homo sapiens L-xylitol + NAD(P)+
-
 r
1.1.1.21 additional information oxidation of alcohols by NADP+ is negligible at 25°C, measurable at 37°C Homo sapiens ?
-
 ?
1.1.1.21 additional information enzyme involved in metabolism of corticosteroids Homo sapiens ?
-
 ?
1.1.1.21 phenylglyoxal + NAD(P)H
-
 Homo sapiens ?
-
 ?
1.1.1.21 xylitol + NAD(P)+
-
 Homo sapiens D-xylose + NAD(P)H + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.21 monomer 1 * 38000, SDS-PAGE Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.21 0.55
-
 D-glyceraldehyde
-
 Homo sapiens
1.1.1.21 0.64
-
 20alpha-Isocorticosterone 20alpha-isocortisol Homo sapiens
1.1.1.21 0.76
-
 Phenylglyoxal
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.21 5.1
-
 90 min, 50% loss of activity Homo sapiens
1.1.1.21 6
-
 unstable below Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.21 NADH 5% Homo sapiens
1.1.1.21 NADPH
-
 Homo sapiens