EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.169 | expression in Escherichia coli BL21(DE3) | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | NADP+ | competitive versus NADPH, noncompetitive versus ketopantoate | Escherichia coli | |
1.1.1.169 | Pantoate | noncompetitive versus ketopantoate and NADPH | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.169 | 0.004 | - |
NADPH | +/-0.0004 | Escherichia coli | |
1.1.1.169 | 0.007 | - |
NADP+ | +/-0.002 | Escherichia coli | |
1.1.1.169 | 0.12 | - |
ketopantoate | +/-0.008 | Escherichia coli | |
1.1.1.169 | 0.26 | - |
Pantoate | +/-0.04 | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | additional information | does not require Mg2+, independent of divalent metal ions | Escherichia coli |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 33000 | - |
SDS-PAGE, gel filtration | Escherichia coli |
1.1.1.169 | 33866 | - |
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
1.1.1.169 | 33870 | - |
1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
1.1.1.169 | 34000 | - |
gel filtration | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | alpha-ketopantoate + NADPH | Escherichia coli | pantothenate/coenzyme A biosynthetic pathway | D-pantoate + NADP+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.169 | Escherichia coli | - |
K12, expressed in BL21(DE3) | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.169 | anion-exchange chromatography, gel filtration chromatography | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.169 | 19 | - |
- |
Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.169 | alpha-ketopantoate + NADPH | B-specific | Escherichia coli | D-pantoate + NADP+ | - |
r | |
1.1.1.169 | alpha-ketopantoate + NADPH | pantothenate/coenzyme A biosynthetic pathway | Escherichia coli | D-pantoate + NADP+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.169 | monomer | 1 * 33000 gel filtration, 1 * 33866 elelectrospray mass spectrometry analysis, 1 * 33870 amino acid sequence | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.169 | NADP+ | - |
Escherichia coli | |
1.1.1.169 | NADPH | - |
Escherichia coli |