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Literature summary extracted from

  • Uehara, K.; Tanimoto, T.; Sato, H.
    Studies on D-tetrose metabolism. IV. Purification and some properties of D-erythrulose reductase from beef liver (1974), J. Biochem., 75, 333-345.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.1.1.162 NADP+ protects against heat inactivation or Rose bengal-sensitized photoinactivation and cold inactivation Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.162 0.0068
-
 NADPH
-
 Bos taurus
1.1.1.162 0.22
-
 NADH
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.162 22000
-
 4 * 22000, SDS-PAGE Bos taurus
1.1.1.162 90000
-
 sedimentation equilibrium analysis, gel filtration, sucrose density gradient centrifugation Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.162 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.162
-
 Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.162 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.162 123
-
-
 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.162 D-erythrulose + NAD(P)H highly specific for D-erythrulose Bos taurus D-threitol + NAD(P)+
-
 ir
1.1.1.162 D-erythrulose + NAD(P)H reversibility could not be demonstrated Bos taurus D-threitol + NAD(P)+
-
 ir
1.1.1.162 D-erythrulose + NADH + H+
-
 Bos taurus D-threitol + NAD+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.162 tetramer 4 * 22000, SDS-PAGE Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.162 additional information
-
 cold inactivation Bos taurus
1.1.1.162
-
-
 23.5 h, 90% loss of activity, regains about 55-65% of its original activity after 60 min Bos taurus
1.1.1.162 21
-
 23.5 h, 20-25% loss of activity Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.162 5.85
-
 D-erythrulose + NADH Bos taurus
1.1.1.162 6.25
-
 D-erythrulose + NADH Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.162 NADH utilized more slowly than NADPH Bos taurus
1.1.1.162 NADPH
-
 Bos taurus