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Literature summary extracted from

  • Sylvester, D.R.; Alvarez, E.; Patel, A.; Ratnam, K.; Kallender, H.; Wallis, N.G.
    Identification and characterization of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from the gram-positive pathogen Streptococcus pneumoniae (2001), Biochem. J., 355, 431-435.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.98 overexpression of protein in Escherichia coli strain BL21(DE3) Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.98 Hg2+
-
 Streptococcus pneumoniae
1.3.1.98 Pb2+
-
 Streptococcus pneumoniae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.98 0.079
-
 NADPH
-
 Streptococcus pneumoniae
1.3.1.98 0.089
-
 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine
-
 Streptococcus pneumoniae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.98 Cs+
-
 Streptococcus pneumoniae
1.3.1.98 K+ without monovalent cations, only minimium enzyme activity Streptococcus pneumoniae
1.3.1.98 K+ K+ causes greatest activation Streptococcus pneumoniae
1.3.1.98 additional information divalent cations have no activating effect Streptococcus pneumoniae
1.3.1.98 additional information no activation with Li+ Streptococcus pneumoniae
1.3.1.98 Na+
-
 Streptococcus pneumoniae
1.3.1.98 NH4+
-
 Streptococcus pneumoniae
1.3.1.98 Rb+
-
 Streptococcus pneumoniae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.98 35000
-
 reducing SDS-PAGE Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Streptococcus pneumoniae
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Streptococcus pneumoniae 100993
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Streptococcus pneumoniae
-
 strain 0100993
-
1.3.1.98 Streptococcus pneumoniae 100993
-
 strain 0100993
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98
-
 Streptococcus pneumoniae

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.1.98 UDP-N-acetyl-alpha-D-muramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-alpha-D-glucosamine + NADPH + H+ ping pong mechanism, contains FAD Streptococcus pneumoniae
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Streptococcus pneumoniae UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Streptococcus pneumoniae 100993 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 FAD absorbance spectrum is characteristic of flavoproteins Streptococcus pneumoniae
1.3.1.98 additional information NADH not substrate Streptococcus pneumoniae
1.3.1.98 additional information thio-NADH, nicotinamide hyopxanthine dinucleotide in reduced form not substrates Streptococcus pneumoniae
1.3.1.98 additional information 3'-NADPH, alpha-NADPH are substrates Streptococcus pneumoniae
1.3.1.98 NADPH
-
 Streptococcus pneumoniae
1.3.1.98 nicotinamide hypoxanthine dinucleotide phosphate reduced form, active substrate Streptococcus pneumoniae