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Literature summary extracted from

  • Taku, A.; Anwar, R.A.
    Biosynthesis of uridine diphospho-N-acetylmuramic acid. IV. Activation of uridine diphospho-N-acetylenolpyruvylglucosamine reductase by monovalent cations (1973), J. Biol. Chem., 248, 4971-4976.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.98 Cs+
-
 Enterobacter cloacae
1.3.1.98 Li+ slight, in presence of K+ Enterobacter cloacae
1.3.1.98 Mg2+ markedly inhibits stimulatory effect of K+ Enterobacter cloacae
1.3.1.98 Mn2+ markedly inhibits stimulatory effect of K+ Enterobacter cloacae
1.3.1.98 N-ethylmaleimide Na+ and K+ provide small but definite protection against inactivation by N-ethylmaleimide Enterobacter cloacae
1.3.1.98 Na+ slight, in presence of K+ Enterobacter cloacae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.3.1.98 K+ one K+ binding site per catalytic center Enterobacter cloacae
1.3.1.98 K+ effect on pH optimum and Km Enterobacter cloacae
1.3.1.98 Li+
-
 Enterobacter cloacae
1.3.1.98 Na+
-
 Enterobacter cloacae
1.3.1.98 NH4+
-
 Enterobacter cloacae
1.3.1.98 Rb+
-
 Enterobacter cloacae
1.3.1.98 Tl+ affinity about 8 times greater than K+, activation in decreasing order: Tl+, K+, NH4+, Rb+, Li+, Na+ Enterobacter cloacae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.98 35500
-
 gel filtration Enterobacter cloacae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH Enterobacter cloacae NRC 492
-
 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.98 Enterobacter cloacae
-
 NRC 492
-
1.3.1.98 Enterobacter cloacae NRC 492
-
 NRC 492
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.98
-
 Enterobacter cloacae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.98 1.84
-
-
 Enterobacter cloacae

Storage Stability

EC Number Storage Stability Organism
1.3.1.98 -20°C, 0.05 M Tris-HCl buffer, pH 8.0, 1 mM dithiothreitol, 30% glycerol, stable for over three months Enterobacter cloacae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Enterobacter cloacae UDP-N-acetyl-3-D-muramate + NADP+
-
 ?
1.3.1.98 UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH
-
 Enterobacter cloacae NRC 492 UDP-N-acetyl-3-D-muramate + NADP+
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.98 8.1 8.5
-
 Enterobacter cloacae

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.98 NADPH
-
 Enterobacter cloacae