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Literature summary extracted from

  • Sueoka, T.; Katoh, S.
    Purification and characterization of sepiapterin reductase from rat erythrocytes (1982), Biochim. Biophys. Acta, 717, 265-271.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.153 Cibacron Blue 3G-A IC50 0.01 mM Rattus norvegicus
1.1.1.153 dihydrobiopterin non-competitive with NADPH or sepiapterin as varying substrate Rattus norvegicus
1.1.1.153 NADP+ competitive inhibition at fixed concentration with varied NADPH, noncompetitive with varied sepiapterin Rattus norvegicus
1.1.1.153 NADPH product inhibition, with more than 0.1 mM, with 0.05 mM sepiapterin Rattus norvegicus
1.1.1.153 sepiapterin product inhibition, with more than 0.05 mM, with 0.1 mM NADPH Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.153 0.0017
-
 NADPH
-
 Rattus norvegicus
1.1.1.153 0.015
-
 sepiapterin
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.153 27500
-
 2 * 27500, SDS-PAGE Rattus norvegicus
1.1.1.153 55000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus equilibrium lies much in favor of dihydrobiopterin formation 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH Rattus norvegicus terminal step in the biosynthetic pathway for tetrahydrobiopterin 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.153 L-erythro-7,8-dihydrobiopterin + NADP+ = sepiapterin + NADPH + H+ ordered bi-bi mechanism, NADPH binds first and NADP+ releases last Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.153 erythrocyte
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.153 18
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
1.1.1.153 -80°C, in 50 mM potassium phosphate buffer, 0,1 M KCl, pH 6.8 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH equilibrium lies much in favor of dihydrobiopterin formation Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?
1.1.1.153 6-(S)-lactoyl-7,8-dihydropterin + NADPH terminal step in the biosynthetic pathway for tetrahydrobiopterin Rattus norvegicus 6-(L-erythro-1',2'-dihydroxypropyl)-7,8-dihydropterin + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.153 homodimer 2 * 27500, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.153 5.5
-
 in potassium phosphate buffer Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.153 5.5 7.4 pH 5.5: optimum, pH 7.4: in 50 mM Tris-HCl buffer about 80% of maximum activity observed Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NADPH
-
 Rattus norvegicus

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.153 0.01
-
 IC50 0.01 mM Rattus norvegicus Cibacron Blue 3G-A