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Literature summary extracted from

  • Yamaguchi, H.; Kanayama, Y.; Yamaki, S.
    Purification and properties of NAD-dependent sorbitol dehydrogenase from apple fruit (1994), Plant Cell Physiol., 35, 887-892.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.14 CaCl2
-
 Malus domestica
1.1.1.14 HgCl2
-
 Malus domestica
1.1.1.14 MgCl2
-
 Malus domestica
1.1.1.14 ZnCl2
-
 Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.14 40.3
-
 D-sorbitol
-
 Malus domestica
1.1.1.14 215
-
 D-fructose
-
 Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.14 120000
-
 gel filtration Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.14 Malus domestica
-
 apple
-
1.1.1.14 Malus domestica
-
 cv. Ourin
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.14 fruit
-
 Malus domestica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.14 1.105
-
-
 Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.14 D-fructose + NADH + H+
-
 Malus domestica D-sorbitol + NAD+
-
 ?
1.1.1.14 D-mannitol + NAD+
-
 Malus domestica ?
-
 ?
1.1.1.14 D-ribitol + NAD+
-
 Malus domestica ?
-
 ?
1.1.1.14 D-sorbitol + NAD+
-
 Malus domestica D-fructose + NADH + H+
-
 ?
1.1.1.14 D-xylitol + NAD+
-
 Malus domestica ?
-
 ?
1.1.1.14 ethanol + NAD+
-
 Malus domestica acetaldehyde + NADH
-
 ?
1.1.1.14 L-arabitol + NAD+ low activity Malus domestica ?
-
 ?
1.1.1.14 L-threitol + NAD+
-
 Malus domestica ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.14 6
-
 D-fructose reduction Malus domestica
1.1.1.14 9.6
-
 sorbitol oxidation Malus domestica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.14 5 7 30% of maximal activity at pH 5.0 and 60% of maximal activity at pH 7.0, fructose reduction Malus domestica
1.1.1.14 9 10.5 less than 70% of maximal activity above and below, sorbitol oxidation Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.14 NAD+
-
 Malus domestica
1.1.1.14 NADH
-
 Malus domestica