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Literature summary extracted from
- Sorbitol dehydrogenase from Bacillus subtilis, purification, characterization and gene cloning (1992), J. Biol. Chem., 267, 24989-24994.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.14 | - |
Bacillus subtilis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.1.1.14 | 11 | - |
D-sorbitol | - |
Bacillus subtilis |  |
| 1.1.1.14 | 14 | - |
D-xylitol | - |
Bacillus subtilis | |
| 1.1.1.14 | 18 | - |
L-Iditol | - |
Bacillus subtilis | |
| 1.1.1.14 | 125 | - |
D-ribitol | - |
Bacillus subtilis | |
| 1.1.1.14 | 500 | - |
D-galactitol | - |
Bacillus subtilis | |
| 1.1.1.14 | 555 | - |
D-mannitol | - |
Bacillus subtilis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.14 | Zn | 1 mol zinc per subunit | Bacillus subtilis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.14 | 39000 | - |
4 * 39000, SDS-PAGE | Bacillus subtilis |
| 1.1.1.14 | 153000 | - |
gel filtration | Bacillus subtilis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.14 | Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.14 | - |
Bacillus subtilis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.14 | 13.6 | - |
- |
Bacillus subtilis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.14 | D-galactitol + NAD+ | - |
Bacillus subtilis | D-tagatose + NADH | - |
? | |
| 1.1.1.14 | D-mannitol + NAD+ | low activity | Bacillus subtilis | ? | - |
? | |
| 1.1.1.14 | D-ribitol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
| 1.1.1.14 | D-sorbitol + NAD+ | - |
Bacillus subtilis | D-fructose + NADH + H+ | - |
? | |
| 1.1.1.14 | D-xylitol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
| 1.1.1.14 | L-iditol + NAD+ | - |
Bacillus subtilis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.1.14 | tetramer | 4 * 39000, SDS-PAGE | Bacillus subtilis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.14 | NAD+ | - |
Bacillus subtilis | |
| 1.1.1.14 | NADH | - |
Bacillus subtilis | |
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