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Literature summary extracted from

  • Adachi, O.; Shinagawa, E.; Matsushita, K.; Ameyama, M.
    Crystallization and properties of 5-keto-D-gluconate reductase from Gluconobacter suboxydans (1979), Agric. Biol. Chem., 43, 75-83.
No PubMed abstract available

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.69
-
 Gluconobacter oxydans
1.1.1.264 55% ammonium sulfate precipitation Gluconobacter oxydans

General Stability

EC Number General Stability Organism
1.1.1.264 highly unstable, 75% loss of activity within a few days, stabilizing agents during purification: D-gluconate, 5-keto-D-gluconate Gluconobacter oxydans

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.69 Hg2+
-
 Gluconobacter oxydans
1.1.1.69 PCMB
-
 Gluconobacter oxydans
1.1.1.264 Cd2+
-
 Gluconobacter oxydans
1.1.1.264 Cs2+
-
 Gluconobacter oxydans
1.1.1.264 Cu2+
-
 Gluconobacter oxydans
1.1.1.264 Hg2+
-
 Gluconobacter oxydans
1.1.1.264 Ni2+
-
 Gluconobacter oxydans
1.1.1.264 p-chloromercuribenzoate
-
 Gluconobacter oxydans
1.1.1.264 Sn2+
-
 Gluconobacter oxydans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.69 0.006
-
 NADPH
-
 Gluconobacter oxydans
1.1.1.69 0.02
-
 NADP+
-
 Gluconobacter oxydans
1.1.1.69 0.9
-
 5-Dehydro-D-gluconate
-
 Gluconobacter oxydans
1.1.1.69 20
-
 D-gluconate
-
 Gluconobacter oxydans
1.1.1.264 0.006
-
 NADPH
-
 Gluconobacter oxydans
1.1.1.264 0.02
-
 NADP+
-
 Gluconobacter oxydans
1.1.1.264 0.9
-
 5-Keto-D-gluconate
-
 Gluconobacter oxydans
1.1.1.264 20
-
 D-gluconate
-
 Gluconobacter oxydans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.264 Mn2+ slight activation at 1 mM Gluconobacter oxydans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.69 25000
-
 4 * 25000, SDS-PAGE Gluconobacter oxydans
1.1.1.264 25000
-
 4 * 25000, SDS-PAGE Gluconobacter oxydans
1.1.1.264 110000
-
 gel filtration Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.69 5-dehydro-D-gluconate + NADPH Gluconobacter oxydans the suggested physiological role of the enzyme is to reduce 5-dehydro-D-gluconate to D-gluconate to supply a carbon source and regenerate NADP+ D-gluconate + NADP+
-
 ?
1.1.1.264 L-idonate + NADP+ Gluconobacter oxydans
-
 5-dehydro-D-gluconate + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.69 Gluconobacter oxydans
-
 IFO 12528
-
1.1.1.264 Gluconobacter oxydans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.69
-
 Gluconobacter oxydans
1.1.1.264 to homogeneity, crystalline, chromatography techniques Gluconobacter oxydans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.264 cell culture
-
 Gluconobacter oxydans
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.69 69.69
-
-
 Gluconobacter oxydans
1.1.1.264 69.7
-
-
 Gluconobacter oxydans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.69 5-dehydro-D-gluconate + NADPH
-
 Gluconobacter oxydans D-gluconate + NADP+
-
 ?
1.1.1.69 5-dehydro-D-gluconate + NADPH the suggested physiological role of the enzyme is to reduce 5-dehydro-D-gluconate to D-gluconate to supply a carbon source and regenerate NADP+ Gluconobacter oxydans D-gluconate + NADP+
-
 ?
1.1.1.264 5-keto-D-fructose + NADP+ low activity Gluconobacter oxydans ? + NADPH
-
 ?
1.1.1.264 D-gluconate + NADP+
-
 Gluconobacter oxydans ? + NADPH
-
 r
1.1.1.264 L-idonate + NADP+
-
 Gluconobacter oxydans 5-dehydro-D-gluconate + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.69 tetramer 4 * 25000, SDS-PAGE Gluconobacter oxydans
1.1.1.264 tetramer 4 * 25000, SDS-PAGE Gluconobacter oxydans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.69 50
-
 reduction of 5-dehydro-D-gluconate and oxidation of D-gluconate Gluconobacter oxydans
1.1.1.264 50
-
-
 Gluconobacter oxydans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.69 additional information
-
 thermal stability is enhanced by addition of either D-gluconate or 5-keto-D-gluconate, or both Gluconobacter oxydans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.69 5.5
-
 reduction of 5-dehydro-D-gluconate Gluconobacter oxydans
1.1.1.69 10
-
 oxidation of D-gluconate in presence of NADP+ Gluconobacter oxydans
1.1.1.264 5.5
-
 5-keto-D-gluconate reduction Gluconobacter oxydans
1.1.1.264 10
-
 D-gluconate oxidation Gluconobacter oxydans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.264 5 6 80% of maximal activity within Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.69 NADPH cofactor Gluconobacter oxydans
1.1.1.264 NADPH
-
 Gluconobacter oxydans