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Literature summary extracted from

  • Tsuji, Y.; Koike, A.; Yamamoto, K.; Tochikura, T.
    Purification and some properties of L-fucose dehydrogenase from Agrobacterium radiobacter and its application to the assay of bound-fucose in glycoconjugates (1992), Biochim. Biophys. Acta, 1117, 167-173.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.122 analysis determination of bound-fucose in biological materials by a coupled enzymatic method in a single buffer system Agrobacterium tumefaciens

General Stability

EC Number General Stability Organism
1.1.1.122 sensitive to repeated freezing and thawing Agrobacterium tumefaciens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.122 Cu2+ complete inhibition at 1 mM Agrobacterium tumefaciens
1.1.1.122 Hg2+ complete inhibition at 1 mM Agrobacterium tumefaciens
1.1.1.122 N-ethylmaleimide strong inhibition at 0.1 mM Agrobacterium tumefaciens
1.1.1.122 p-chloromercuribenzoate strong inhibition at 0.1 mM Agrobacterium tumefaciens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.122 0.02
-
 NADP+
-
 Agrobacterium tumefaciens
1.1.1.122 0.61
-
 NAD+
-
 Agrobacterium tumefaciens
1.1.1.122 3
-
 6-deoxy-L-galactose
-
 Agrobacterium tumefaciens
1.1.1.122 14.3
-
 D-arabinose
-
 Agrobacterium tumefaciens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.122 28000
-
 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens
1.1.1.122 37000
-
 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens
1.1.1.122 64000
-
 gel filtration Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ Agrobacterium tumefaciens i.e. L-fucose 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.122 D-arabinose + NAD+ Agrobacterium tumefaciens 71% of the activity compared to L-fucose D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 L-galactose + NAD+ Agrobacterium tumefaciens 57% of the activity compared to L-fucose L-galactono-1,5-lactone + NADH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.122 Agrobacterium tumefaciens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.122 to homogeneity, chromatography techniques Agrobacterium tumefaciens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.122 cell culture
-
 Agrobacterium tumefaciens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.122 53
-
 purified enzyme Agrobacterium tumefaciens

Storage Stability

EC Number Storage Stability Organism
1.1.1.122 -30°C, 6 months Agrobacterium tumefaciens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.122 6-deoxy-L-galactose + NAD+ i.e. L-fucose Agrobacterium tumefaciens 6-deoxy-L-galactono-1,5-lactone + NADH
-
 ?
1.1.1.122 D-arabinose + NAD+ 71% of the activity compared to L-fucose Agrobacterium tumefaciens D-arabinono-1,4-lactone + NADH
-
 ?
1.1.1.122 fuconolactone + NADPH + H+
-
 Agrobacterium tumefaciens L-fucose + NADP+
-
 ?
1.1.1.122 L-galactose + NAD+ 57% of the activity compared to L-fucose Agrobacterium tumefaciens L-galactono-1,5-lactone + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.122 dimer 1 * 37000 + 1 * 28000, SDS-PAGE Agrobacterium tumefaciens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.122 45
-
 stable up to Agrobacterium tumefaciens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.122 7 9 active within Agrobacterium tumefaciens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.122 7.5 9.5 stable within Agrobacterium tumefaciens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.122 NAD+ also able to use NADP+ Agrobacterium tumefaciens
1.1.1.122 NADP+ 30% of the activity compared to NAD+, L-fucose dehydrogenation Agrobacterium tumefaciens