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Literature summary extracted from

  • Weinhold, E.G.; Benner, S.A.
    Engineering yeast alcohol dehydrogenase. Replacing Trp54 by Leu broadens substrate specificity (1995), Protein Eng., 8, 457-461.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.1 W54L less active than the wild-type enzyme with ethanol, 1-propanol and 1-butanol. With 1-pentanol and 1-hexanol the mutant enzyme is a better catalyst than the wild-type enzyme Equus caballus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 1.5
-
 cinnamyl alcohol mutant enzyme W54L Equus caballus
1.1.1.1 4
-
 ethanol wild-type enzyme Adh1 Equus caballus
1.1.1.1 4.58
-
 cinnamyl alcohol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 6.5
-
 Hexanol mutant enzyme W54L Equus caballus
1.1.1.1 6.7
-
 4-Methyl-1-pentanol mutant enzyme W54L Equus caballus
1.1.1.1 11.8
-
 Pentanol mutant enzyme W54L Equus caballus
1.1.1.1 13.4
-
 ethanol mutant enzyme W54L Equus caballus
1.1.1.1 13.6
-
 Propanol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 13.8
-
 Hexanol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 29.4
-
 butanol mutant enzyme W54L Equus caballus
1.1.1.1 43.9
-
 Propanol mutant enzyme W54L Equus caballus
1.1.1.1 53.8
-
 4-Methyl-1-pentanol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 53.9
-
 butanol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 56.2
-
 Pentanol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 93.3
-
 butan-2-ol wilde-type enzyme ADh1 Equus caballus
1.1.1.1 193
-
 propan-2-ol mutant enzyme W54L Equus caballus
1.1.1.1 268
-
 2-propanol wilde-type enzyme ADh1 Equus caballus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Equus caballus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Equus caballus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.1 liver
-
 Equus caballus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 3-methyl-1-butanol + NAD+
-
 Equus caballus ? + NADH
-
 ?
1.1.1.1 4-methyl-1-pentanol + NAD+
-
 Equus caballus 4-methyl-1-pentanal + NADH
-
 ?
1.1.1.1 butan-2-ol + NAD+
-
 Equus caballus butan-2-one + NADH
-
 ?
1.1.1.1 cinnamyl alcohol + NAD+
-
 Equus caballus cinnamaldehyde + NADH
-
 ?
1.1.1.1 ethanol + NAD+
-
 Equus caballus acetaldehyde + NADH
-
 ?
1.1.1.1 hexanol + NAD+
-
 Equus caballus n-hexanal + NADH
-
 ?
1.1.1.1 pentanal + NAD+
-
 Equus caballus pentanone + NADH
-
 ?
1.1.1.1 propan-2-ol + NAD+
-
 Equus caballus acetone + NADH
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.1 0.08
-
 3-methyl-1-butanol mutant enzyme W54L Equus caballus
1.1.1.1 0.27
-
 3-methyl-1-butanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 0.31
-
 butan-2-ol mutant enzyme W54L Equus caballus
1.1.1.1 5.8
-
 propan-2-ol mutant enzyme W54L Equus caballus
1.1.1.1 6.7
-
 4-Methyl-1-pentanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 11.9
-
 butan-2-ol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 18.4
-
 Pentanol mutant enzyme W54L Equus caballus
1.1.1.1 18.6
-
 4-Methyl-1-pentanol mutant enzyme W54L Equus caballus
1.1.1.1 23.9
-
 Hexanol mutant enzyme W54L Equus caballus
1.1.1.1 28.7
-
 Propanol mutant enzyme W54L Equus caballus
1.1.1.1 29.6
-
 Hexanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 32.7
-
 butanol mutant enzyme W54L Equus caballus
1.1.1.1 53.1
-
 propan-2-ol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 56.2
-
 Pentanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 98
-
 cinnamyl alcohol mutant enzyme W54L Equus caballus
1.1.1.1 102
-
 ethanol mutant enzyme W54L Equus caballus
1.1.1.1 118
-
 butanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 123
-
 ethanol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 133
-
 cinnamyl alcohol wild-type enzyme Adh 1 Equus caballus
1.1.1.1 308
-
 ethanol wild-type enzyme Adh 1 Equus caballus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Equus caballus
1.1.1.1 NADH
-
 Equus caballus