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Literature summary extracted from

  • Ammendola, S.; Raia, C.A.; Caruso, C.; Camardella, L.; D'Auria, S.; De Rosa, M.; Rossi, M.
    Thermostable NAD(+)-dependent alcohol dehydrogenase from Sulfolobus solfataricus: gene and protein sequence determination and relationship to other alcohol dehydrogenases (1992), Biochemistry, 31, 12514-12523.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.1 0.036
-
 NADH
-
 Saccharolobus solfataricus
1.1.1.1 0.09
-
 Anisaldehyde
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.1 Zinc
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 37000
-
 2 * 37000, SDS-PAGE Saccharolobus solfataricus
1.1.1.1 74000
-
 gel filtration, sucrose density gradient centrifugation Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Saccharolobus solfataricus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.1
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.1 5.3
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 anisaldehyde + NADH + H+
-
 Saccharolobus solfataricus anisic alcohol + NAD+
-
 ?
1.1.1.1 benzyl alcohol + NAD+
-
 Saccharolobus solfataricus benzaldehyde + NADH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.1 dimer 2 * 37000, SDS-PAGE Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.1 65
-
 pH 8.0, protein concentration 2.5 mg/ml, 16 h, stable. 50% loss of activity after 8 h at protein concentration of 0.5 mg/ml Saccharolobus solfataricus
1.1.1.1 85
-
 pH 8.0, protein concentration 0.5 mg/ml, 3 h, 50% loss of activity Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Saccharolobus solfataricus
1.1.1.1 NADH
-
 Saccharolobus solfataricus