BRENDA - Enzyme Database show

Isolation and characterization of pig kidney mitochondrial ferredoxin:NADP+ oxidoreductase

Gnanaiah, W.; Omdahl, J.L.; J. Biol. Chem. 261, 12649-12654 (1986)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.00094
-
NADPH
-
Sus scrofa
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.18.1.2
mitochondrion
-
Sus scrofa
5739
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.2
48000
-
SDS-PAGE
Sus scrofa
1.18.1.2
51500
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.2
NADPH + oxidized ferredoxin
Sus scrofa
supports in vivo reduction of membrane bound adrenal mitochondrial P-450
NADP+ + reduced ferredoxin
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.18.1.2
Sus scrofa
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.18.1.2
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.1.2
16.3
-
reduction of cytochrome c
Sus scrofa
Storage Stability
EC Number
Storage Stability
Organism
1.18.1.2
4°C, stable for 1 week at 4°C when bound to ADP-agarose column
Sus scrofa
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
additional information
-
285507
Sus scrofa
?
-
-
-
-
1.18.1.2
NADPH + oxidized ferredoxin
-
285507
Sus scrofa
NADP+ + reduced ferredoxin
-
285507
Sus scrofa
?
1.18.1.2
NADPH + oxidized ferredoxin
supports in vivo reduction of membrane bound adrenal mitochondrial P-450
285507
Sus scrofa
NADP+ + reduced ferredoxin
-
-
-
-
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.2
FAD
flavoprotein
Sus scrofa
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.2
FAD
flavoprotein
Sus scrofa
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.2
0.00094
-
NADPH
-
Sus scrofa
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.18.1.2
mitochondrion
-
Sus scrofa
5739
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.18.1.2
48000
-
SDS-PAGE
Sus scrofa
1.18.1.2
51500
-
gel filtration
Sus scrofa
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.2
NADPH + oxidized ferredoxin
Sus scrofa
supports in vivo reduction of membrane bound adrenal mitochondrial P-450
NADP+ + reduced ferredoxin
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.18.1.2
kidney
-
Sus scrofa
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.18.1.2
16.3
-
reduction of cytochrome c
Sus scrofa
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.18.1.2
4°C, stable for 1 week at 4°C when bound to ADP-agarose column
Sus scrofa
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.18.1.2
additional information
-
285507
Sus scrofa
?
-
-
-
-
1.18.1.2
NADPH + oxidized ferredoxin
-
285507
Sus scrofa
NADP+ + reduced ferredoxin
-
285507
Sus scrofa
?
1.18.1.2
NADPH + oxidized ferredoxin
supports in vivo reduction of membrane bound adrenal mitochondrial P-450
285507
Sus scrofa
NADP+ + reduced ferredoxin
-
-
-
-