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Literature summary extracted from
- Ecdysone 20-monooxygenase systems in flesh-flies (diptera: sarcophagidae), Neobellieria bullata and Parasarcophaga argyrostoma (1993), Comp. Biochem. Physiol. B, 105, 765-773.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.22 | diquat dibromide | - |
Parasarcophaga argyrostoma |  |
| 1.14.99.22 | diquat dibromide | - |
Sarcophaga bullata | |
| 1.14.99.22 | Tween 80 | microsomal isozyme | Parasarcophaga argyrostoma | |
| 1.14.99.22 | Tween 80 | microsomal isozyme | Sarcophaga bullata | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.99.22 | microsome | microsomal isozyme | Sarcophaga bullata | - |
- |
| 1.14.99.22 | microsome | microsomal isozyme | Parasarcophaga argyrostoma | - |
- |
| 1.14.99.22 | mitochondrion | mitochondrial isozyme | Sarcophaga bullata | 5739 | - |
| 1.14.99.22 | mitochondrion | mitochondrial isozyme | Parasarcophaga argyrostoma | 5739 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.22 | ecdysone + NADPH + O2 | Sarcophaga bullata | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| 1.14.99.22 | ecdysone + NADPH + O2 | Parasarcophaga argyrostoma | - |
20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.99.22 | Parasarcophaga argyrostoma | - |
flesh-fly | - |
| 1.14.99.22 | Sarcophaga bullata | - |
flesh-fly | - |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima | Sarcophaga bullata | |
| 1.14.99.22 | ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O | 2 different isozymes of mitochondria and microsomes may have different inducers, electron donors, endogenous inhibitors, and physiological optima | Parasarcophaga argyrostoma |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.99.22 | body wall | - |
Sarcophaga bullata | - |
| 1.14.99.22 | body wall | - |
Parasarcophaga argyrostoma | - |
| 1.14.99.22 | fat body | third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body | Sarcophaga bullata | - |
| 1.14.99.22 | fat body | third instar wandering stage larvae 67-68% of total activity, after white prepuparial stage activity is inactivated in fat body | Parasarcophaga argyrostoma | - |
| 1.14.99.22 | malpighian tubule | - |
Sarcophaga bullata | - |
| 1.14.99.22 | malpighian tubule | - |
Parasarcophaga argyrostoma | - |
| 1.14.99.22 | ring gland | - |
Sarcophaga bullata | - |
| 1.14.99.22 | ring gland | - |
Parasarcophaga argyrostoma | - |
| 1.14.99.22 | trachea | 8-9% of total activity | Sarcophaga bullata | - |
| 1.14.99.22 | trachea | 8-9% of total activity | Parasarcophaga argyrostoma | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 0.00000000617 | - |
fat body | Parasarcophaga argyrostoma |
| 1.14.99.22 | 0.0000000103 | - |
fat body | Sarcophaga bullata |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.99.22 | alpha-ecdysone + NADPH + O2 | - |
Sarcophaga bullata | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| 1.14.99.22 | alpha-ecdysone + NADPH + O2 | - |
Parasarcophaga argyrostoma | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| 1.14.99.22 | ecdysone + NADPH + O2 | - |
Sarcophaga bullata | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
| 1.14.99.22 | ecdysone + NADPH + O2 | - |
Parasarcophaga argyrostoma | 20-hydroxyecdysone + NADP+ + H2O | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 30 | - |
microsomal isozyme | Sarcophaga bullata |
| 1.14.99.22 | 30 | - |
microsomal isozyme | Parasarcophaga argyrostoma |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.99.22 | 7.2 | - |
microsomal isozyme | Sarcophaga bullata |
| 1.14.99.22 | 7.2 | - |
microsomal isozyme | Parasarcophaga argyrostoma |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.99.22 | cytochrome P450 | essential | Sarcophaga bullata | |
| 1.14.99.22 | cytochrome P450 | essential | Parasarcophaga argyrostoma | |
| 1.14.99.22 | Ferredoxin | isozyme in mitochondria | Sarcophaga bullata | |
| 1.14.99.22 | Ferredoxin | isozyme in mitochondria | Parasarcophaga argyrostoma | |
| 1.14.99.22 | NADPH | dependent on | Sarcophaga bullata | |
| 1.14.99.22 | NADPH | dependent on | Parasarcophaga argyrostoma | |
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