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Literature summary extracted from

  • Weirich, G.F.
    Ecdysone 20-monooxygenase (1985), Methods Enzymol., 3, 454-458.
No PubMed abstract available

General Stability

EC Number General Stability Organism
1.14.99.22 bovine serum albumin stabilizes Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.22 0.00183
-
 ecdysone mitochondrial enzyme system Manduca sexta

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.22 ecdysone + NADPH + O2 Manduca sexta serves as precursor of ecdysterone, the active moulting hormone in larval insects 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 ecdysone + NADPH + O2 Manduca sexta alpha-ecdysone, a secretory product of prothoracic glands 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 ecdysone + NADPH + O2 Manduca sexta last step in biosynthesis of insect molting hormone 20-hydroxyecdysone + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.22 Manduca sexta
-
 tobacco hornworm
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.22
-
 Manduca sexta

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.99.22 ecdysone + reduced acceptor + O2 = 20-hydroxyecdysone + acceptor + H2O NADPH-dependent monooxygenase containing cytochrome P-450, a heme-thiolate protein Manduca sexta
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.99.22 0.0000466
-
 mitochondrial enzyme system Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.22 alpha-ecdysone + NADPH + O2
-
 Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 ecdysone + NADPH + O2 serves as precursor of ecdysterone, the active moulting hormone in larval insects Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 ecdysone + NADPH + O2 alpha-ecdysone, a secretory product of prothoracic glands Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
 ?
1.14.99.22 ecdysone + NADPH + O2 last step in biosynthesis of insect molting hormone Manduca sexta 20-hydroxyecdysone + NADP+ + H2O
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.99.22 cytochrome P450 essential Manduca sexta
1.14.99.22 NADPH
-
 Manduca sexta