EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | methyltrienolone | synthetic androgen, used as photoaffinity ligand and substrate analog, covalent binding, 0.1 mM inhibits cortisol synthesis, during photolabeling radioactivity incorporation via radioactive methyltrienolone is blocked by 11-deoxycorticosterone, so it binds to the conserved substrate binding region Trp428-Leu429-Asp430-Arg431 between beta3-sheet and the L-helix analysed by trypsin digest | Bos taurus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.15.4 | 0.0008 | - |
11-deoxycorticosterone | presence of methyltrienolone | Bos taurus | |
1.14.15.4 | 0.002 | - |
11-deoxycorticosterone | absence of methyltrienolone | Bos taurus | |
1.14.15.4 | 0.06 | 0.09 | corticosterone | presence of methyltrienolone | Bos taurus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.14.15.4 | mitochondrion | - |
Bos taurus | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.15.4 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.15.4 | RP HPLC for detection of photolabeled peptide after digest with TPCK-treated trypsin of purified cytochrome P-45011beta, which is photolabeled with radioactive methyltrienolone | Bos taurus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.14.15.4 | a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O | superfamily of heme-containing enzyme, 11-/18-/19-hydroxylations of 11-deoxycorticosterone, 11-deoxycortisol and androstenedione | Bos taurus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.14.15.4 | adrenal cortex | - |
Bos taurus | - |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.15.4 | -80°C, 20 mM potassium phosphate buffer, pH 7.4, 0.01 mM 11-deoxycorticosterone, 0.1 mM EDTA, 0.1 mM dithiothreitol, 1%, w/v, sodium cholate | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.15.4 | 11-deoxycortisol + reduced adrenal ferredoxin + O2 | cytochrome P-45011beta-linked monoxygenase | Bos taurus | cortisol + oxidized adrenal ferredoxin + H2O | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.15.4 | cytochrome P-45011-beta | - |
Bos taurus |
1.14.15.4 | P-450XIB1 | - |
Bos taurus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 37 | - |
assay at | Bos taurus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.15.4 | 7.4 | - |
assay at | Bos taurus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.15.4 | NADPH | adrenodoxin and NADPH-linked adrenodoxin reductase | Bos taurus |