BRENDA - Enzyme Database show

Properties of an adrenal cytochrome P-450 (P-45011beta) for the hydroxylations of corticosteroids

Sato, H.; Ashida, N.; Suhara, K.; Itagaki, E.; Takemori, S.; Katagiri, M.; Arch. Biochem. Biophys. 190, 307-314 (1978)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.4
CO
affects 11beta-/19-hydroxylase reaction
Bos taurus
1.14.15.4
Metyrapone
competitive with substrate, affects strongly 11beta-/18-hydroxylation and 11beta-/19-hydroxylation
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.4
0.006
-
deoxycorticosterone
identical for both enzyme activities
Bos taurus
1.14.15.4
0.013
-
4-androstene-3,17-dione
identical for both enzyme activities
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.4
mitochondrion
-
Bos taurus
5739
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.4
Bos taurus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.14.15.4
copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction
Bos taurus
Reaction
EC Number
Reaction
Commentary
Organism
1.14.15.4
a steroid + 2 reduced adrenodoxin + O2 + 2 H+ = an 11beta-hydroxysteroid + 2 oxidized adrenodoxin + H2O
P-45011beta responsible for two hydroxylase reactions
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.4
adrenal cortex
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.4
additional information
-
activities of sonicated mitochondria and cholate extract of hydroxylation activities with deoxycotricosterone and 4-androstene-3,17-dione as substrates
Bos taurus
Storage Stability
EC Number
Storage Stability
Organism
1.14.15.4
-80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity
285391
Bos taurus
18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity
285391
Bos taurus
corticosterone + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2
i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate
285391
Bos taurus
11beta-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2
i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate
285391
Bos taurus
19-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.4
37
-
assay at
Bos taurus
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.15.4
0.3
-
11-deoxycorticosterone
18-hydroxylation
Bos taurus
1.14.15.4
1.83
-
11-deoxycorticosterone
11beta-hydroxylation
Bos taurus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.4
7.4
-
assay at
Bos taurus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.4
NADPH
-
Bos taurus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.15.4
NADPH
-
Bos taurus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.15.4
CO
affects 11beta-/19-hydroxylase reaction
Bos taurus
1.14.15.4
Metyrapone
competitive with substrate, affects strongly 11beta-/18-hydroxylation and 11beta-/19-hydroxylation
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.15.4
0.006
-
deoxycorticosterone
identical for both enzyme activities
Bos taurus
1.14.15.4
0.013
-
4-androstene-3,17-dione
identical for both enzyme activities
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.15.4
mitochondrion
-
Bos taurus
5739
-
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.4
copurification of 11beta-/18-hydroxylase with 11beta-/19-hydroxylase, cholate extraction
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.15.4
adrenal cortex
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.14.15.4
additional information
-
activities of sonicated mitochondria and cholate extract of hydroxylation activities with deoxycotricosterone and 4-androstene-3,17-dione as substrates
Bos taurus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.14.15.4
-80°C, 59 mM potassium phosphate buffer, pH 7.4, 0.1 mM EDTA, 0.1 mM dithiothreitol, 0.01 mM deoxycorticosterone, 0.3% cholate, 0.3% Tween 20
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity
285391
Bos taurus
18-hydroxycorticosterone + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
11-deoxycorticosterone + reduced adrenal ferredoxin + O2
containing adrenal ferredoxin, adrenal ferredoxin reductase and P-45011beta, highly active hydroxylation, 11beta-/18-hydroxylase activity
285391
Bos taurus
corticosterone + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2
i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate
285391
Bos taurus
11beta-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
1.14.15.4
4-androstene-3,17-dione + reduced adrenal ferredoxin + O2
i.e. testosterone, 11beta-/19-hydroxylase activity, good sustrate
285391
Bos taurus
19-hydroxy-4-androstene-3,17-dione + oxidized adrenal ferredoxin + H2O
-
285391
Bos taurus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.15.4
37
-
assay at
Bos taurus
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.14.15.4
0.3
-
11-deoxycorticosterone
18-hydroxylation
Bos taurus
1.14.15.4
1.83
-
11-deoxycorticosterone
11beta-hydroxylation
Bos taurus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.14.15.4
7.4
-
assay at
Bos taurus