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Literature summary extracted from

  • McFarlane, I.J.; Lees, E.M
    Conn, E.E.: The in vitro biosynthesis of dhurrin, the cyanogenic glycoside of Sorghum bicolor (1975), J. Biol. Chem., 250, 4708-4713.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.36 microsome
-
 Sorghum bicolor
-
-
1.14.14.37 microsome
-
 Sorghum bicolor
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.36 Sorghum bicolor
-
-
-
1.14.14.37 Sorghum bicolor
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.36 seedling
-
 Sorghum bicolor
-
1.14.14.37 seedling
-
 Sorghum bicolor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.14.37 0.003
-
 conversion of p-hydroxyphenylacetaldoxime to p-hydrobenzaldehyde Sorghum bicolor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.36 L-tyrosine + 2 O2 + 2 [reduced NADPH-hemoprotein reductase]
-
 Sorghum bicolor (E)-[4-hydroxyphenylacetaldehyde oxime] + 2 [oxidized NADPH-hemoprotein reductase] + CO2 + 3 H2O
-
 ?
1.14.14.37 (E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?
1.14.14.37 (E)-4-hydroxyphenylacetaldehyde oxime + [reduced NADPH-hemoprotein reductase] + O2
-
 Sorghum bicolor (S)-4-hydroxymandelonitrile + [oxidized NADPH-hemoprotein reductase] + 2 H2O overall reaction ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.37 7.8 8.4 formation of p-hydroxybenzaldehyde from L-tyrosine in microsomal fractions Sorghum bicolor

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.37 NADH less effective than NADPH Sorghum bicolor
1.14.14.37 NADPH
-
 Sorghum bicolor
1.14.14.37 O2
-
 Sorghum bicolor

General Information

EC Number General Information Comment Organism
1.14.14.36 physiological function a microsomal fraction from seedlings of Sorghum bicolor catalyzes the conversion of L-tyrosine to 4-hydroxymandelonitrile via 4-hydroxyphenylacetaldoxime Sorghum bicolor
1.14.14.37 physiological function a microsomal fraction from seedlings of Sorghum bicolor catalyzes the conversion of L-tyrosine to 4-hydroxymandelonitrile via 4-hydroxyphenylacetaldoxime Sorghum bicolor