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Literature summary extracted from
- Microsomal isoflavone 2'- and 3'-hydroxylases from chickpea (Cicer arietinum L.) cell suspensions induced for pterocarpan phytoalexin formation (1987), FEBS Lett., 214, 101-106.
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.88 | additional information | cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity | Cicer arietinum | |
| 1.14.14.88 | NADH | synergistic effect with NADPH, 150% of NADPH activity | Cicer arietinum |  |
| 1.14.14.89 | additional information | cells susceptible to Ascochyta rabiei can be elicitor-induced to 500% activity | Cicer arietinum | |
| 1.14.14.89 | NADH | synergistic effect with NADPH | Cicer arietinum | |
| 1.14.14.89 | NADH | 135% of NADPH activity | Cicer arietinum | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.88 | cytochrome c | 0.01 mM reduces activity to 4%, 0.1 mM reduces activity to 1% | Cicer arietinum | |
| 1.14.14.88 | additional information | no inhibition by 5 mM KCN and 1 mM EDTA | Cicer arietinum | |
| 1.14.14.89 | cytochrome c | 0.01 mM cytochrome c reduces activity to 14%, 0.1 mM cytochrome c reduces activity to 4% | Cicer arietinum | |
| 1.14.14.89 | additional information | no inhibition by 5 mM KCN and 1 mM EDTA | Cicer arietinum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.14.88 | microsome | - |
Cicer arietinum | - |
- |
| 1.14.14.89 | microsome | - |
Cicer arietinum | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.88 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | Cicer arietinum | involved in the biosynthesis of the pterocarpin phytoalexin maackiain, cytochrome P450-dependent monooxygenase | 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | Cicer arietinum | involved in the biosynthesis of the pterocarpin phytoalexin medicarpin | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | Cicer arietinum | cytochrome P450-dependent monooxygenase | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.14.88 | Cicer arietinum | - |
- |
- |
| 1.14.14.89 | Cicer arietinum | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.14.88 | cell suspension culture | - |
Cicer arietinum | - |
| 1.14.14.89 | cell culture | suspension | Cicer arietinum | - |
| 1.14.14.89 | cell suspension culture | - |
Cicer arietinum | - |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 1.14.14.88 | -20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h | Cicer arietinum |
| 1.14.14.89 | -20°C, 100 mM potassium phosphate, pH 7.5 containing 400 mM sucrose and 3.5 mM 2-mercaptoethanol, 24 h | Cicer arietinum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.14.88 | biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | pratensein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.88 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.88 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | involved in the biosynthesis of the pterocarpin phytoalexin maackiain, cytochrome P450-dependent monooxygenase | Cicer arietinum | 3'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.88 | additional information | no substrates are genistein and daidzein | Cicer arietinum | ? | - |
? | |
| 1.14.14.88 | additional information | monohydroxylation of 4'-methoxyisoflavones | Cicer arietinum | ? | - |
? | |
| 1.14.14.89 | biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.89 | biochanin A + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxybiochanin A + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | daidzein + [reduced NADPH-hemoprotein reductase] + O2 | 2.0% of the activity with biochanin A | Cicer arietinum | 2'-hydroxydaidzein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | - |
Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | involved in the biosynthesis of the pterocarpin phytoalexin medicarpin | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | cytochrome P450-dependent monooxygenase | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
ir | |
| 1.14.14.89 | formononetin + [reduced NADPH-hemoprotein reductase] + O2 | 78.1% of the activity with biochanin A | Cicer arietinum | 2'-hydroxyformononetin + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.89 | genistein + [reduced NADPH-hemoprotein reductase] + O2 | 2.2% of the activity with biochanin A | Cicer arietinum | 2'-hydroxygenistein + [oxidized NADPH-hemoprotein reductase] + H2O | - |
? | |
| 1.14.14.89 | additional information | monohydroxylation of 4'-methoxyisoflavones, no substrates are genistein and daidzein | Cicer arietinum | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.14.89 | isoflavone 2'-hydroxylase | ambiguous | Cicer arietinum |
| 1.14.14.89 | isoflavone 2'-monooxygenase | ambiguous | Cicer arietinum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.14.88 | 7.5 | - |
assay conditions | Cicer arietinum |
| 1.14.14.89 | 7.5 | - |
assay conditions | Cicer arietinum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.14.88 | additional information | no cofactors are FAD and FMN | Cicer arietinum | |
| 1.14.14.89 | additional information | no cofactors are FAD and FMN | Cicer arietinum |
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