BRENDA - Enzyme Database show

Manganese lipoxygenase. Purification and characterization

Su, C.; Oliw, E.H.; J. Biol. Chem. 273, 13072-13079 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.45
arachidonic acid
inhibits at 0.01 mM
Gaeumannomyces graminis
1.13.11.45
eicosatetraynoic acid
50% of control activity at 0.01 mM
Gaeumannomyces graminis
1.13.11.45
additional information
no inhibition by lipoxygenase inhibitors zileuton, esculetin and ICI 230487
Gaeumannomyces graminis
1.13.11.45
N-(3-phenoxycinnamyl)acetohydroxamic acid
50% of control activity at 0.05 mM
Gaeumannomyces graminis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.45
0.0024
-
alpha-linolenic acid
-
Gaeumannomyces graminis
1.13.11.45
0.0044
-
linoleic acid
-
Gaeumannomyces graminis
1.13.11.45
0.031
-
O2
-
Gaeumannomyces graminis
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.45
Mn2+
contains 0.5-1.0 atom per enzyme molecule
Gaeumannomyces graminis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.45
73000
-
SDS-PAGE after deglycosylation
Gaeumannomyces graminis
1.13.11.45
135000
-
gel filtration with Superdex 200 in 6 M urea
Gaeumannomyces graminis
1.13.11.45
160000
-
gel filtration with Superdex 200
Gaeumannomyces graminis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.45
Gaeumannomyces graminis
-
take-all fungus
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
1.13.11.45
glycoprotein
-
Gaeumannomyces graminis
Purification (Commentary)
EC Number
Commentary
Organism
1.13.11.45
-
Gaeumannomyces graminis
Storage Stability
EC Number
Storage Stability
Organism
1.13.11.45
4C, 3 mM NaN3, 0.2 mM GSH, several months, no loss of activity
Gaeumannomyces graminis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.45
alpha-linolenic acid + O2
-
285253
Gaeumannomyces graminis
(9Z,11E,15Z)-(13)-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.45
gamma-linolenic acid + O2
-
285253
Gaeumannomyces graminis
(6Z,9Z,11E)-(13)-hydroperoxyoctadecatrienoic acid + (6Z,9Z,12Z)-(11)-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.45
linoleic acid + O2
-
285253
Gaeumannomyces graminis
(9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.45
60
-
-
Gaeumannomyces graminis
Temperature Range [C]
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.45
12
60
8.8% of maximal activity at 12C and 100% of maximal activity at 60C, activity decreases sharply at 63C
Gaeumannomyces graminis
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.45
63
-
incubation for 5 min over 63C decreases activity, no activity at 75C
Gaeumannomyces graminis
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.45
18.3
-
linoleic acid
-
Gaeumannomyces graminis
1.13.11.45
23.3
-
O2
-
Gaeumannomyces graminis
1.13.11.45
40
-
alpha-linolenic acid
-
Gaeumannomyces graminis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.45
7
-
broad pH optimum
Gaeumannomyces graminis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.45
5
11
at pH 5.0 64% of maximum activity, at pH 11 69% of maximum activity
Gaeumannomyces graminis
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.13.11.45
arachidonic acid
inhibits at 0.01 mM
Gaeumannomyces graminis
1.13.11.45
eicosatetraynoic acid
50% of control activity at 0.01 mM
Gaeumannomyces graminis
1.13.11.45
additional information
no inhibition by lipoxygenase inhibitors zileuton, esculetin and ICI 230487
Gaeumannomyces graminis
1.13.11.45
N-(3-phenoxycinnamyl)acetohydroxamic acid
50% of control activity at 0.05 mM
Gaeumannomyces graminis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.13.11.45
0.0024
-
alpha-linolenic acid
-
Gaeumannomyces graminis
1.13.11.45
0.0044
-
linoleic acid
-
Gaeumannomyces graminis
1.13.11.45
0.031
-
O2
-
Gaeumannomyces graminis
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.13.11.45
Mn2+
contains 0.5-1.0 atom per enzyme molecule
Gaeumannomyces graminis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.13.11.45
73000
-
SDS-PAGE after deglycosylation
Gaeumannomyces graminis
1.13.11.45
135000
-
gel filtration with Superdex 200 in 6 M urea
Gaeumannomyces graminis
1.13.11.45
160000
-
gel filtration with Superdex 200
Gaeumannomyces graminis
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
1.13.11.45
glycoprotein
-
Gaeumannomyces graminis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.45
-
Gaeumannomyces graminis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
1.13.11.45
4C, 3 mM NaN3, 0.2 mM GSH, several months, no loss of activity
Gaeumannomyces graminis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.45
alpha-linolenic acid + O2
-
285253
Gaeumannomyces graminis
(9Z,11E,15Z)-(13)-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.45
gamma-linolenic acid + O2
-
285253
Gaeumannomyces graminis
(6Z,9Z,11E)-(13)-hydroperoxyoctadecatrienoic acid + (6Z,9Z,12Z)-(11)-hydroperoxyoctadecatrienoic acid
-
-
-
?
1.13.11.45
linoleic acid + O2
-
285253
Gaeumannomyces graminis
(9Z,11E)-(13R)-hydroperoxyoctadecadienoic acid
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.13.11.45
60
-
-
Gaeumannomyces graminis
Temperature Range [C] (protein specific)
EC Number
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
1.13.11.45
12
60
8.8% of maximal activity at 12C and 100% of maximal activity at 60C, activity decreases sharply at 63C
Gaeumannomyces graminis
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
1.13.11.45
63
-
incubation for 5 min over 63C decreases activity, no activity at 75C
Gaeumannomyces graminis
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.13.11.45
18.3
-
linoleic acid
-
Gaeumannomyces graminis
1.13.11.45
23.3
-
O2
-
Gaeumannomyces graminis
1.13.11.45
40
-
alpha-linolenic acid
-
Gaeumannomyces graminis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.13.11.45
7
-
broad pH optimum
Gaeumannomyces graminis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.13.11.45
5
11
at pH 5.0 64% of maximum activity, at pH 11 69% of maximum activity
Gaeumannomyces graminis