BRENDA - Enzyme Database

Phosphatidylinositol phosphodiesterase in higher plants

Irvine, R.F.; Letcher, A.J.; Dawson, R.M.C.; Biochem. J. 192, 279-283 (1980)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
phosphatidic acid
stimulating
Apium graveolens Dulce Group
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
EDTA
addition of EDTA completely inhibits the activity
Apium graveolens Dulce Group
3.1.4.11
additional information
dialysis of the Apium graveolens var. dulce extract against water completely inhibits the activity
Apium graveolens Dulce Group
3.1.4.11
phosphatidylcholine
slightly inhibited, 30% at concentrations equimolar with phosphatidylinositol
Apium graveolens Dulce Group
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
soluble
-
Apium graveolens Dulce Group
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
enzyme requires a bivalent cation, Ca2+ being the most effective activator
Apium graveolens Dulce Group
3.1.4.11
Mg2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
3.1.4.11
Mn2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
3.1.4.11
Zn2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.4.11
Allium cepa
-
-
-
3.1.4.11
Apium graveolens Dulce Group
-
celery
-
3.1.4.11
Brassica oleracea var. botrytis
-
cauliflower
-
3.1.4.11
Narcissus pseudonarcissus
-
daffodil
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
flower
tips of the flowers
Brassica oleracea var. botrytis
-
3.1.4.11
leaf
outer leaves
Allium cepa
-
3.1.4.11
stem
the lower regions of the stem
Apium graveolens Dulce Group
-
3.1.4.11
stem
the elongating stem
Narcissus pseudonarcissus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
additional information
-
at a substrate concentration of 50 microM the rate of hydrolysis is 0.3 nmol/h per mg of tissue (wet weight) at pH 6.6
Apium graveolens Dulce Group
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
additional information
no detectable activity against phosphatidylcholine or phosphatidylethanolamine
285228
Apium graveolens Dulce Group
?
-
-
-
-
3.1.4.11
phosphatidylinositol + H2O
-
285228
Apium graveolens Dulce Group
diacylglycerol + 1D-myo-inositol 1-phosphate
the lipoidal product of hydrolysis is diacylglycerol, the water-soluble products are 1D-myo-inositol 1,2-cyclic phosphate and 1D-myo-inositol 1-phosphate in the approximate proportions of 60% and 40%, respectively
-
-
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
5.9
6.6
-
Apium graveolens Dulce Group
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.4.11
phosphatidic acid
stimulating
Apium graveolens Dulce Group
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.4.11
EDTA
addition of EDTA completely inhibits the activity
Apium graveolens Dulce Group
3.1.4.11
additional information
dialysis of the Apium graveolens var. dulce extract against water completely inhibits the activity
Apium graveolens Dulce Group
3.1.4.11
phosphatidylcholine
slightly inhibited, 30% at concentrations equimolar with phosphatidylinositol
Apium graveolens Dulce Group
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.4.11
soluble
-
Apium graveolens Dulce Group
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.4.11
Ca2+
enzyme requires a bivalent cation, Ca2+ being the most effective activator
Apium graveolens Dulce Group
3.1.4.11
Mg2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
3.1.4.11
Mn2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
3.1.4.11
Zn2+
calcium, manganese, magnesium and zinc ions, in decreasing order of effectiveness, all partly restore the activity of the dialysed enzyme at a concentration of 2 mM. In each case 4 mM cation is less effective than 2 mM
Apium graveolens Dulce Group
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.4.11
flower
tips of the flowers
Brassica oleracea var. botrytis
-
3.1.4.11
leaf
outer leaves
Allium cepa
-
3.1.4.11
stem
the lower regions of the stem
Apium graveolens Dulce Group
-
3.1.4.11
stem
the elongating stem
Narcissus pseudonarcissus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.4.11
additional information
-
at a substrate concentration of 50 microM the rate of hydrolysis is 0.3 nmol/h per mg of tissue (wet weight) at pH 6.6
Apium graveolens Dulce Group
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.4.11
additional information
no detectable activity against phosphatidylcholine or phosphatidylethanolamine
285228
Apium graveolens Dulce Group
?
-
-
-
-
3.1.4.11
phosphatidylinositol + H2O
-
285228
Apium graveolens Dulce Group
diacylglycerol + 1D-myo-inositol 1-phosphate
the lipoidal product of hydrolysis is diacylglycerol, the water-soluble products are 1D-myo-inositol 1,2-cyclic phosphate and 1D-myo-inositol 1-phosphate in the approximate proportions of 60% and 40%, respectively
-
-
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.4.11
5.9
6.6
-
Apium graveolens Dulce Group