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Literature summary extracted from

  • Char, S.; Gopinathan, K.P.
    Arginyl-tRNA synthetase from Mycobacterium smegmatis SN2: purification and kinetic mechanism (1986), J. Biochem., 100, 349-357.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.19 polyamines stimulate the formation of arginyl-tRNAArg, the stimulation being more significant at sub-optimal Mg2+ concentrations Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.19 AMP
-
 Mycolicibacterium smegmatis
6.1.1.19 diphosphate
-
 Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.19 0.0001 0.0002 tRNAArg
-
 Mycolicibacterium smegmatis
6.1.1.19 0.0006
-
 Arg
-
 Mycolicibacterium smegmatis
6.1.1.19 0.4
-
 tubercidin 5'-triphosphate ATP Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.19 Mg2+ required Mycolicibacterium smegmatis
6.1.1.19 Mg2+ optimal concentration is 6 mM Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.19 55000
-
 1 * 55000, SDS-PAGE Mycolicibacterium smegmatis
6.1.1.19 58000
-
 gel filtration Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.19 Mycolicibacterium smegmatis
-
-
-
6.1.1.19 Mycolicibacterium smegmatis SN2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.19
-
 Mycolicibacterium smegmatis

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.19 ATP + L-arginine + tRNAArg = AMP + diphosphate + L-arginyl-tRNAArg rapid equilibrium random ter ter mechanism Mycolicibacterium smegmatis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.19 0.00703
-
-
 Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.19 tubercidin 5'-triphosphate + L-arginine + tRNAArg
-
 Mycolicibacterium smegmatis tubercidin 5'-monophosphate + diphosphate + L-arginyl-tRNAArg
-
 ?
6.1.1.19 tubercidin 5'-triphosphate + L-arginine + tRNAArg
-
 Mycolicibacterium smegmatis SN2 tubercidin 5'-monophosphate + diphosphate + L-arginyl-tRNAArg
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.19 monomer 1 * 55000, SDS-PAGE Mycolicibacterium smegmatis