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Literature summary extracted from

  • Moriyasu, Y.; Sakano, K.; Tazawa, M.
    Vacuolar/extravacuolar distribution of aminopeptidase in giant alga Chara australis and partial purification of one such enzyme (1987), Plant Physiol., 84, 720-725.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.22 1,10 phenanthroline
-
Chara australis
3.4.11.22 EDTA
-
Chara australis
3.4.11.22 iodoacetate
-
Chara australis
3.4.11.22 additional information no inhibition by diisopropyl fluorophosphate, phenylmethylsulfonyl fluoride Chara australis
3.4.11.22 N-tosyl-L-Phe chloromethyl ketone slightly inhibitory Chara australis
3.4.11.22 p-chloromercuribenzoate
-
Chara australis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.22 additional information extravacuolar, , 60% of enzyme activity Chara australis
-
-
3.4.11.22 vacuole 40% of enzyme activity Chara australis 5773
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.22 44000
-
x * 62000 + x * 44000, SDS-PAGE Chara australis
3.4.11.22 62000
-
x * 62000 + x * 44000, SDS-PAGE Chara australis
3.4.11.22 120000
-
gel permeation chromatography Chara australis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.22 Chara australis
-
giant alga
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.22 partially Chara australis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.22 whole plant
-
Chara australis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.22 6.36
-
-
Chara australis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.22 Ala 2-naphthylamide + H2O
-
Chara australis Ala + 2-naphthylamine
-
?
3.4.11.22 Arg 2-naphthylamide + H2O
-
Chara australis Arg + 2-naphthylamine
-
?
3.4.11.22 Asp 2-naphthylamide + H2O very slight activity Chara australis Asp + 2-naphthylamine
-
?
3.4.11.22 Leu 2-naphthylamide + H2O
-
Chara australis Leu + 2-naphthylamine
-
?
3.4.11.22 Lys 2-naphthylamide + H2O
-
Chara australis Lys + 2-naphthylamine
-
?
3.4.11.22 additional information no hydrolyis of N-alpha-benzoyl-DL-Arg 2-naphthylamide Chara australis ?
-
?
3.4.11.22 Phe 2-naphthylamide + H2O
-
Chara australis Phe + 2-naphthylamine
-
?
3.4.11.22 Pro 2-naphthylamide + H2O
-
Chara australis Pro + 2-naphthylamine
-
?
3.4.11.22 Ser 2-naphthylamide + H2O
-
Chara australis Ser + 2-naphthylamine
-
?
3.4.11.22 Tyr 2-naphthylamide + H2O
-
Chara australis Tyr + 2-naphthylamine
-
?
3.4.11.22 Val 2-naphthylamide + H2O slight activity Chara australis Val + 2-naphthylamine
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.22 ? x * 62000 + x * 44000, SDS-PAGE Chara australis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.22 6
-
substrate Leu 2-naphthylamide Chara australis
3.4.11.22 7 8 substrate Ala 2-naphthylamide Chara australis