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Literature summary extracted from

  • Danno, G.
    Studies on the D-glucose-isomerizing enzyme from Bacillus coagulans, strain HN-68. Part V. Comparative study on the three activities of D-glucose, D-xylose and D-ribose isomerization of the crystalline enzyme (1970), Agric. Biol. Chem., 34, 1805-1814.
No PubMed abstract available

General Stability

EC Number General Stability Organism
5.3.1.5 Mn2+, Co2+ and Ni2+ strongly protect the enzyme from heat denaturation Weizmannia coagulans

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.3.1.5 D-mannitol competitive Weizmannia coagulans
5.3.1.5 D-ribose inhibits D-glucose isomerization Weizmannia coagulans
5.3.1.5 D-sorbitol competitive Weizmannia coagulans
5.3.1.5 D-xylitol competitive Weizmannia coagulans
5.3.1.5 D-xylose inhibits D-glucose isomerization Weizmannia coagulans
5.3.1.5 EDTA
-
 Weizmannia coagulans
5.3.1.5 Tris(hydroxymethyl)aminomethane strong inhibition of D-glucose isomerization, slight inhibition of D-xylose isomerization, competitive Weizmannia coagulans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.5 1.1
-
 D-xylose
-
 Weizmannia coagulans
5.3.1.5 77
-
 D-ribose
-
 Weizmannia coagulans
5.3.1.5 90
-
 D-glucose
-
 Weizmannia coagulans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.3.1.5 Co2+ most effectively activates D-glucose isomerization and D-ribose isomerization Weizmannia coagulans
5.3.1.5 Mg2+ activates D-glucose isomerization, D-xylose isomerization and D-ribose isomerization Weizmannia coagulans
5.3.1.5 Mn2+ most effectively activates D-xylose isomerization Weizmannia coagulans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.1.5 D-Xylose Weizmannia coagulans
-
 ?
-
 ?
5.3.1.5 D-Xylose Weizmannia coagulans HN-68
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.5 Weizmannia coagulans
-
-
-
5.3.1.5 Weizmannia coagulans HN-68
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.5 D-Glucose
-
 Weizmannia coagulans D-Fructose
-
 ?
5.3.1.5 D-Glucose
-
 Weizmannia coagulans HN-68 D-Fructose
-
 ?
5.3.1.5 D-Ribose
-
 Weizmannia coagulans ?
-
 ?
5.3.1.5 D-Ribose
-
 Weizmannia coagulans HN-68 ?
-
 ?
5.3.1.5 D-Xylose
-
 Weizmannia coagulans ?
-
 ?
5.3.1.5 D-Xylose
-
 Weizmannia coagulans HN-68 ?
-
 ?
5.3.1.5 D-Xylose
-
 Weizmannia coagulans D-Xylulose
-
 ?
5.3.1.5 D-Xylose
-
 Weizmannia coagulans HN-68 D-Xylulose
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.3.1.5 additional information
-
 Mn2+, Co2+ and Ni2+ strongly protect the enzyme from heat denaturation Weizmannia coagulans
5.3.1.5 50
-
 1 h, stable Weizmannia coagulans
5.3.1.5 70
-
-
 Weizmannia coagulans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.3.1.5 7
-
 D-glucose Weizmannia coagulans
5.3.1.5 7
-
 D-ribose Weizmannia coagulans
5.3.1.5 8 8.5 D-xylose Weizmannia coagulans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.3.1.5 4
-
 50°C, 60 min, complete inactivation below Weizmannia coagulans
5.3.1.5 4.5
-
 50°C, 60 min, 20% loss of activity Weizmannia coagulans
5.3.1.5 5 7 50°C, 60 min, stable Weizmannia coagulans
5.3.1.5 9
-
 50°C, 60 min, 20% loss of activity Weizmannia coagulans